Team:Harvard/parts

From 2010.igem.org

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<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ectysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr>
<tr><tr><td></td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ectysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr>
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<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="">amiRNA LUT2</a></td></tr>
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<tr><td>F5</td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet.  191 amino acid sequence</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr>
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<tr><td>F5</td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet.  191 amino acid sequence</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr>
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<tr><td>F4</td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein.  54 amino acid sequence. </td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr>
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<tr><td>F4</td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein.  54 amino acid sequence. </td><td>kan (bacteria) / nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr>
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<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="">amiRNA LUT2</a></td></tr>
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="">amiRNA LUT2</a></td></tr>
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="">amiRNA LUT2</a></td></tr>
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<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr>
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<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr>
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr>
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr>

Revision as of 23:41, 26 October 2010



parts

Retrieved from "http://2010.igem.org/Team:Harvard/parts"
namebiobrick nameoriginal namedescriptionresistance (bacteria)resistance (plants)sequence
V7BBa_K382000pORE Open Series Vector with BioBrick Sites (kan/pat)Agrobacterium vector open series with biobrick cloning siteKanamycinpat V7
V8BBa_K382001pORE Open Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector open series with biobrick cloning siteKanamycinnptII V8
V9BBa_K382002pORE Expression Series Vector with BioBrick Sites (kan/pat)Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpat V9
V10BBa_K382003pORE Expression Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptII V10
V11BBa_K382004pORE Reporter Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptII V11
V12BBa_K382005pORE Reporter Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptII V12
C1BBa_K382010amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownchloramphenicol-amiRNA LUT2
C2BBa_K382011amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownchloramphenicol-amiRNA BETA-OHASE 1
C3BBa_K382012amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownchloramphenicol-amiRNA LYC
A2BBa_K382017amiRNA LTP LTP knockdown constructampicillin- amiRNA LTP
A3BBa_K382014ihpRNA BetIntron hairpin Bet Knockdown constructampicillin- ihpRNA Bet1
A4BBa_K382015ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol-ihpRNA LTP
A4BBa_K382018PDK intron hairpinIntron hairpin constructampicillin-
A5BBa_K382019ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol-ihpRNA GER
F1BBa_K382022pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants. chloramphenicol-link
F2BBa_K382023NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.chloramphenicol-link
F3BBa_K382024NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on 5' end.chloramphenicol-link
F4BBa_K382020BrazzeinSweet-tasting protein. 54 amino acid sequence. chloramphenicol-link
F5BBa_K382021MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencechloramphenicol-link
F6BBa_K382040Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
F7BBa_K382041Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
F8BBa_K382028Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
F9BBa_K382027Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
GF3BBa_K382035LacINLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoterchloramphenicol-link
GF4BBa_K382031RXRLc Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozideampicillin-link
GF5BBa_K382030RXRHmHuman Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.chloramphenicol-link
BBa_K382033Ecdysone Receptor Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ectysone receptor binds RXR-VP16 in presence of Methoxyfenozide chloramphenicol-link
F5BBa_K382050MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencekan (bacteria)/pat (plant)-link
F5BBa_K382051MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencekan (bacteria)/nptII (plant)-link
F4BBa_K382052BrazzeinSweet-tasting protein. 54 amino acid sequence. kan (bacteria)/pat (plant)-link
F4BBa_K382053BrazzeinSweet-tasting protein. 54 amino acid sequence. kan (bacteria) / nptII (plant)-link
BBa_K382054amiRNA GFPamiRNA construct for GFP knockdownkan (bacteria)/pat (plant)-amiRNA LUT2
BBa_K382055amiRNA GFPamiRNA construct for GFP knockdownkan (bacteria)/nptII (plant)-amiRNA LUT2
BBa_K382058amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownkan (bacteria)/pat (plant)-amiRNA LUT2
BBa_K382059amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownkan (bacteria)/pat (plant)-amiRNA BETA-OHASE 1
BBa_K382060amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownkan (bacteria)/pat (plant)-amiRNA LYC
BBa_K382061amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownkan (bacteria)/nptII (plant)-amiRNA LUT2
BBa_K382062amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownkan (bacteria)/nptII (plant)-amiRNA BETA-OHASE 1
BBa_K382063amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownkan (bacteria)/nptII (plant)-amiRNA LYC
BBa_K382064amiRNA LTP LTP knockdown constructkan (bacteria)/pat (plant)- amiRNA LTP
BBa_K382065amiRNA LTP LTP knockdown constructkan (bacteria)/nptII (plant)- amiRNA LTP
BBa_K382066ihpRNA Ger Intron hairpin Ger Knockdown constructkan (bacteria)/pat (plant)-ihpRNA GER
BBa_K382067ihpRNA Ger Intron hairpin Ger Knockdown constructkan (bacteria)/nptII (plant)-ihpRNA GER
BBa_K382068ihpRNA BetV1Intron hairpin Bet Knockdown constructkan (bacteria)/pat (plant)- ihpRNA Bet1
BBa_K382069ihpRNA BetV1Intron hairpin Bet Knockdown constructkan (bacteria)/nptII (plant)- ihpRNA Bet1
BBa_K382070ihpRNA LTPIntron hairpin LTP Knockdown constructkan (bacteria)/pat (plant)-ihpRNA LTP
BBa_K382071ihpRNA LTPIntron hairpin LTP Knockdown constructkan (bacteria)/nptII (plant)-ihpRNA LTP