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	''pour plates when warm to the touch (about 50°C)''		''pour plates when warm to the touch (about 50°C)''
		+
		+	'''0.1 M CaCl2'''
		+	*X g of CaCl2
		+	*X mL of DI water
		+	''filter sterilize''
	'''Ampicillin Antibiotic Stock Solution (100 mg/mL)'''		'''Ampicillin Antibiotic Stock Solution (100 mg/mL)'''

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Revision as of 17:00, 7 July 2010

Media Recipes

LB media

• 10 g LB broth
• 500 mL DI water

autoclave to sterilize

LB media with agar

• 10 g LB broth
• 7.5 g agar
• 500 mL DI water

autoclave to sterilize

add antibiotics after autoclaving when warm to touch (about 50°C)

pour plates when warm to the touch (about 50°C)

0.1 M CaCl2

• X g of CaCl2
• X mL of DI water

filter sterilize

Ampicillin Antibiotic Stock Solution (100 mg/mL)

• 1 g of ampicillin
• 5 mL of DI water
• 5 mL of ethanol

filter sterilize and make 1 mL aliquotes to store in the -20°C freezer

Kanamycin Antibiotic Stock Solution (50 mg/mL)

• 250 mg of kanamycin
• 5 mL of DI water

filter sterilize and make 1 mL aliquots to store in the -20°C freezer

Tetracycline Antibiotic Stock Solution (50 mg/mL)

• 250 mg of tetracycline
• 2.5 mL of DI water
• 2.5 mL of ethanol

filter sterilize and make 1 mL aliquots to store in the -20°C freezer