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Team:Michigan/Media Recipes
From 2010.igem.org
(Difference between revisions)
| Line 25: | Line 25: | ||
''pour plates when warm to the touch (about 50°C)'' | ''pour plates when warm to the touch (about 50°C)'' | ||
| - | ''' | + | '''Ampicillin Antibiotic Stock Solution (100 mg/mL)''' |
| + | *1 g of ampicillin | ||
| + | *5 mL of DI water | ||
| + | *5 mL of ethanol | ||
| + | ''filter sterilize and make 1 mL aliquotes to store in the -20°C freezer'' | ||
'''Kanamycin Antibiotic Stock Solution (50 mg/mL)''' | '''Kanamycin Antibiotic Stock Solution (50 mg/mL)''' | ||
Revision as of 16:58, 7 July 2010
Media Recipes
LB media
- 10 g LB broth
- 500 mL DI water
autoclave to sterilize
LB media with agar
- 10 g LB broth
- 7.5 g agar
- 500 mL DI water
autoclave to sterilize
add antibiotics after autoclaving when warm to touch (about 50°C)
pour plates when warm to the touch (about 50°C)
Ampicillin Antibiotic Stock Solution (100 mg/mL)
- 1 g of ampicillin
- 5 mL of DI water
- 5 mL of ethanol
filter sterilize and make 1 mL aliquotes to store in the -20°C freezer
Kanamycin Antibiotic Stock Solution (50 mg/mL)
- 250 mg of kanamycin
- 5 mL of DI water
filter sterilize and make 1 mL aliquots to store in the -20°C freezer
Tetracycline Antibiotic Stock Solution (50 mg/mL)
- 250 mg of tetracycline
- 2.5 mL of DI water
- 2.5 mL of ethanol
filter sterilize and make 1 mL aliquots to store in the -20°C freezer
