Team:Wisconsin-Madison/parts

From 2010.igem.org

(Difference between revisions)
(Final Constructs)
(Testing Constructs)
Line 89: Line 89:
|Produces Trascription Factor YgiV - Blocks Biofilm
|Produces Trascription Factor YgiV - Blocks Biofilm
|Inducible - IPTG
|Inducible - IPTG
-
|503
+
|DNA Not Sent
|-
|-
|<partinfo>BBa_k318504</partinfo>
|<partinfo>BBa_k318504</partinfo>
|Produces RfaI gene - WaaI Ligase
|Produces RfaI gene - WaaI Ligase
|Inducible - IPTG
|Inducible - IPTG
-
|504
+
|DNA Not Sent
|-
|-
|<partinfo>BBa_k318505</partinfo>
|<partinfo>BBa_k318505</partinfo>
|Produces T.F. YgiV & RfaI gene
|Produces T.F. YgiV & RfaI gene
|Inducible - IPTG
|Inducible - IPTG
-
|505
+
|DNA Not Sent
|-
|-
|<partinfo>BBa_k318506</partinfo>
|<partinfo>BBa_k318506</partinfo>
Line 109: Line 109:
|Produces Repressor cI-LVA (degredation tag)
|Produces Repressor cI-LVA (degredation tag)
|Inducible - IPTG
|Inducible - IPTG
-
|509
+
|DNA Not Sent
|-
|-
|<partinfo>BBa_k318510</partinfo>
|<partinfo>BBa_k318510</partinfo>
|Produces Repressor cI (no degredation tag)
|Produces Repressor cI (no degredation tag)
|Inducible - IPTG
|Inducible - IPTG
-
|510
+
|DNA Not Sent
|-
|-
|<partinfo>BBa_k318511</partinfo>
|<partinfo>BBa_k318511</partinfo>

Revision as of 23:29, 26 October 2010

New Standards

Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following:

  • Genbank including features and plasmid (.gbk)
  • Fwd and Reverse sequencing files (.ab1)
  • Gels from cloning and confirmation (.pdf)
  • All associated testing files and Results (.xls, .pdf, etc.)

Enzyme Delivery

New DNA

Enzyme Delivery Testing Constructs
Part Number About Testing in... Zip File
<partinfo>BBa_k318512</partinfo> Regulatory - pH inducible promoter <partinfo>BBa_k318513</partinfo> 512
<partinfo>BBa_k318514</partinfo> Gene - RamA protein can bind to cholic acid (bile salts) <partinfo>BBa_k318515</partinfo> Media:Zip_file_ramA.zip
<partinfo>BBa_k318516</partinfo> Regulatory - RamA/Bile-salt conjugate inducible promoter <partinfo>BBa_k318517</partinfo> Media:Zip_file_acrRA.zip


Testing Constructs

Enzyme Delivery Testing Constructs
Part Number Function Expression Type Zip File
<partinfo>BBa_k318500</partinfo> Produces Trascription Factor RcsB Inducible - IPTG 500
<partinfo>BBa_k318501</partinfo> Produces Trascription Factor RcsA Inducible - IPTG 501
<partinfo>BBa_k318502</partinfo> Produces Trascription Factor RcsA & RcsB Inducible - IPTG 502
<partinfo>BBa_k318503</partinfo> Produces Trascription Factor YgiV - Blocks Biofilm Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318504</partinfo> Produces RfaI gene - WaaI Ligase Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318505</partinfo> Produces T.F. YgiV & RfaI gene Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318506</partinfo> Produces RFP (+)LuxR, (-)cILVA 506
<partinfo>BBa_k318509</partinfo> Produces Repressor cI-LVA (degredation tag) Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318510</partinfo> Produces Repressor cI (no degredation tag) Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318511</partinfo> Produces Activator LuxR Anhydrous Tetrycycline 511
<partinfo>BBa_k318513</partinfo> Produces RFP pH 513
<partinfo>BBa_k318515</partinfo> Produces RFP RamA/Bile-Salt Complex Media:Zip_file_acrRA.zip
<partinfo>BBa_k318517</partinfo> Produces RamA gene Bile Salt Media:Zip_file_ramA.zip


Final Constructs

Enzyme Delivery Final Constructs
Part Number Function Expression Type
Not Cloned Produces Trascription Factors RcsA & RcsB, and B-Gal Constitutive
Not Cloned A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA) pH 2
<partinfo>BBa_k318507</partinfo> A) Activates Lysis once in the small intestine (+)-LuxR, (-)-cILVA
<partinfo>BBa_k318519</partinfo> B) Activates lysis once in the small intestine Bile Salts


Encryption

Encryption Constructs
Part Number Description Notes Zip File
<partinfo>BBa_K318000</partinfo> Key Cassette - produces Cre and Hin recombinases Arabinose induces Cre expression, IPTG induces Hin expression Media:BBa_K318000_pSB1C3.zip
<partinfo>BBa_K318030</partinfo> Lock Cassette - prevents transcription unless "unlocked" Requires successive Hin and Cre recombination for constitutive expression of downstream gene(s) Media:BBa_K318030_pSB1C3.zip


Other Parts

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318010 BBa_K318010]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318011 BBa_K318011]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318012 BBa_K318012]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318013 BBa_K318013]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318014 BBa_K318014]