Team:Harvard/parts

From 2010.igem.org

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<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr>
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr>
<tr><tr><td>GF7</td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ectysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr>
<tr><tr><td>GF7</td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ectysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr>
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<tr><td>A4</td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr>
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<tr><td>A4</td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr>

Revision as of 23:23, 26 October 2010



parts

Retrieved from "http://2010.igem.org/Team:Harvard/parts"
namebiobrick nameoriginal namedescriptionresistance (bacteria)resistance (plants)sequence
V7BBa_K382000pORE Open Series Vector with BioBrick Sites (kan/pat)Agrobacterium vector open series with biobrick cloning siteKanamycinpat V7
V8BBa_K382001pORE Open Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector open series with biobrick cloning siteKanamycinnptII V8
V9BBa_K382002pORE Expression Series Vector with BioBrick Sites (kan/pat)Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpat V9
V10BBa_K382003pORE Expression Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptII V10
V11BBa_K382004pORE Reporter Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptII V11
V12BBa_K382005pORE Reporter Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptII V12
C1BBa_K382010amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownchloramphenicol-amiRNA LUT2
C2BBa_K382011amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownchloramphenicol-amiRNA BETA-OHASE 1
C3BBa_K382012amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownchloramphenicol-amiRNA LYC
A2BBa_K382017amiRNA LTP LTP knockdown constructampicillin- amiRNA LTP
A3BBa_K382014ihpRNA BetIntron hairpin Bet Knockdown constructampicillin- ihpRNA Bet1
A4BBa_K382015ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol-ihpRNA LTP
A4BBa_K382018PDK intron hairpinIntron hairpin constructampicillin-
A5BBa_K382019ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol-ihpRNA GER
F1BBa_K382022pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants. chloramphenicol-link
F2BBa_K382023NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.chloramphenicol-link
F3BBa_K382024NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on 5' end.chloramphenicol-link
F4BBa_K382020BrazzeinSweet-tasting protein. 54 amino acid sequence. chloramphenicol-link
F5BBa_K382021MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencechloramphenicol-link
F6BBa_K382040Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
F7BBa_K382041Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
F8BBa_K382028Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
F9BBa_K382027Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
GF3BBa_K382035LacINLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoterchloramphenicol-link
GF4BBa_K382031RXRLc Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozideampicillin-link
GF5BBa_K382030RXRHmHuman Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.chloramphenicol-link
GF7BBa_K382033Ecdysone Receptor Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ectysone receptor binds RXR-VP16 in presence of Methoxyfenozide chloramphenicol-link
A4BBa_K382071ihpRNA LTPIntron hairpin LTP Knockdown constructkan (bacteria)/pat (plant)-ihpRNA LTP
A4BBa_K382071ihpRNA LTPIntron hairpin LTP Knockdown constructkan (bacteria)/nptII (plant)-ihpRNA LTP