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Team:Tsinghua/Notebook/15 September 2010
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(New page: Since it seems as if no ligation product of three fragments appeared in the PCR identification result, we strongly doubt that there is something wrong with the DraIII cutting site, perhaps...)
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(New page: Since it seems as if no ligation product of three fragments appeared in the PCR identification result, we strongly doubt that there is something wrong with the DraIII cutting site, perhaps...)
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Revision as of 21:49, 26 October 2010
Since it seems as if no ligation product of three fragments appeared in the PCR identification result, we strongly doubt that there is something wrong with the DraIII cutting site, perhaps there lies mutaion site. To confirm our proposal, we decide to conduct the ligation sepratatively and then PCR is run.
