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Team:Tsinghua/Notebook/26 August 2010
From 2010.igem.org
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== Module I, group 2(b) == | == Module I, group 2(b) == | ||
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The PEMKC 3-5 are positive. We’ll wait for the sequence result to ensure. | The PEMKC 3-5 are positive. We’ll wait for the sequence result to ensure. | ||
| + | == Module I, Group 2c == | ||
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| + | Ligation product is transformed in to DH5aα 24h after ligation. | ||
Revision as of 20:27, 26 October 2010
Module I, group 2(b)
Run a colony PCR with universal primers. Amplify the bacteria at the same time.
PCR system (SuperMix):
H2O 8μl primer1 1μl primer2 1μl template Some SuperMix 10μl Total 20μl
result
The PEMKC 3-5 are positive. We’ll wait for the sequence result to ensure.
Module I, Group 2c
Ligation product is transformed in to DH5aα 24h after ligation.
