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Team:Tsinghua/Notebook/20 August 2010
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(New page: == Module I, group 2c == Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positive one. digestion system ...)
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(New page: == Module I, group 2c == Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positive one. digestion system ...)
Newer edit →
Revision as of 19:10, 26 October 2010
Module I, group 2c
Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positive one.
digestion system
plasmid 6ul EcoRI 1ul FD Buffer 2ul H2O 11ul
Result: None of the clone picked is positive.
