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| | {{:Team:Queens-Canada/head}} | | {{:Team:Queens-Canada/head}} |
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| - | =Part Structure=
| + | <h1>WormWorks Parts List</h1> |
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| | <html><script type="text/javascript"> | | <html><script type="text/javascript"> |
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| | <div class="box"> | | <div class="box"> |
| | <html><a href="#" onclick="toggle_visibility('groupparts');">Show/hide parts</a></html> as entered in the [http://partsregistry.org Registry]. | | <html><a href="#" onclick="toggle_visibility('groupparts');">Show/hide parts</a></html> as entered in the [http://partsregistry.org Registry]. |
| - | <div id="groupparts" style="display: none;"> | + | <div id="groupparts" style="display: block;"> |
| | <groupparts>iGEM010 Queens-Canada</groupparts> | | <groupparts>iGEM010 Queens-Canada</groupparts> |
| | </div> | | </div> |
| | </div> | | </div> |
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| - | '''Note:''' The following list is tentative, and better reflects our goals rather than our current accomplishments. We are still in the process of isolating and characterising these components.
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| - |
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| - | =General=
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| - |
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| - | ==Promoters==
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| - |
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| - | {|
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| - | !Name
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| - | !Description
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| - | !Strength
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| - | |-
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| - | |''sip-1''
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| - | |Heat shock
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| - | |
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| - | |-
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| - | |''rab-7''
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| - | |Constitutive
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| - | |high
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| - | |-
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| - | |''mec-7''
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| - | |Mechanosensory neurons
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| - | |
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| - | |-
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| - | |''hsp-3''
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| - | |Heat shock
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| - | |
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| - | |-
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| - | |''gpd-2''
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| - | |Constitutive
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| - | |very high
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| - | |-
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| - | |''unc-47''
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| - | |GABA-using (gabergic) neurons
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| - | |
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| - | |}
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| - |
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| - | ==Coding Sequences==
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| - |
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| - | {|
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| - | !Name
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| - | !Description
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| - | |-
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| - | |GFP
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| - | |Enhanced Green Fluorescent Protein. From biobrick BBa_I714891. Proof of concept to see if genes can be imported from bacteria.
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| - | |-
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| - | |eGFP
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| - | |Enhanced Green Fluorescent Protein. From Chin-Sang plasmid pIC691.
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| - | |-
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| - | |mCherry
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| - | |Red Fluorescent Protein. From Chin-Sang plasmid.
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| - | |-
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| - | |YFP
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| - | |Yellow Fluorescent Protein. From Chin-Sang plasmid.
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| - | |-
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| - | |eCFP
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| - | |Enhanced Cyan Fluorescent Protein. From Chin-Sang plasmid.
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| - | |}
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| - |
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| - | ==Backbones==
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| - |
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| - | * Since it is possible to microinject plasmids into ''C. elegans'', we're working on a combination backbone that will produce GFP in both ''E. coli'' and ''C. elegans'', as well as providing antibiotic selection markers for ''E. coli'', and the cutsites from Standard 10.
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| - | * Another project we're pursuing is the possible usage of double-stranded RNA as a liaison for DNA import. If this works, we'll have hybrid strand backbones for that method of transformation as well.
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| - |
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| - | =Optogenetics (R.''C. elegans'')=
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| - |
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| - | ==Promoters==
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| - |
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| - | {|
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| - | !Name
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| - | !Description
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| - | !Strength
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| - | |-
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| - | |}
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| - |
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| - | ==Coding Squences==
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| - |
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| - | {|
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| - | !Name
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| - | !Description
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| - | |-
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| - | |ChR2
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| - | |Channelrhodopsin-2. Permits entry of positive ions in response to 460 nm light. Can be used to trigger nerve cells.
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| - | |-
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| - | |NpHR
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| - | |Halorhodopsin. Permits entry of chloride ions in response to 580 nm light. Can be used to block nerve cells from firing.
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| - | |}
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| | | | |
| | {{:Team:Queens-Canada/foot}} | | {{:Team:Queens-Canada/foot}} |
WormWorks Parts List
Show/hide parts as entered in the [http://partsregistry.org Registry].
<groupparts>iGEM010 Queens-Canada</groupparts>
"
