Team:Queens-Canada/parts

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
{{:Team:Queens-Canada/head}}
{{:Team:Queens-Canada/head}}
-
=Part Structure=
+
<h1>WormWorks Parts List</h1>
<html><script type="text/javascript">
<html><script type="text/javascript">
Line 15: Line 15:
<div class="box">
<div class="box">
<html><a href="#" onclick="toggle_visibility('groupparts');">Show/hide parts</a></html> as entered in the [http://partsregistry.org Registry].
<html><a href="#" onclick="toggle_visibility('groupparts');">Show/hide parts</a></html> as entered in the [http://partsregistry.org Registry].
-
<div id="groupparts" style="display: none;">
+
<div id="groupparts" style="display: block;">
<groupparts>iGEM010 Queens-Canada</groupparts>
<groupparts>iGEM010 Queens-Canada</groupparts>
</div>
</div>
</div>
</div>
-
 
-
'''Note:''' The following list is tentative, and better reflects our goals rather than our current accomplishments. We are still in the process of isolating and characterising these components.
 
-
 
-
=General=
 
-
 
-
==Promoters==
 
-
 
-
{|
 
-
!Name
 
-
!Description
 
-
!Strength
 
-
|-
 
-
|''sip-1''
 
-
|Heat shock
 
-
|
 
-
|-
 
-
|''rab-7''
 
-
|Constitutive
 
-
|high
 
-
|-
 
-
|''mec-7''
 
-
|Mechanosensory neurons
 
-
|
 
-
|-
 
-
|''hsp-3''
 
-
|Heat shock
 
-
|
 
-
|-
 
-
|''gpd-2''
 
-
|Constitutive
 
-
|very high
 
-
|-
 
-
|''unc-47''
 
-
|GABA-using (gabergic) neurons
 
-
|
 
-
|}
 
-
 
-
==Coding Sequences==
 
-
 
-
{|
 
-
!Name
 
-
!Description
 
-
|-
 
-
|GFP
 
-
|Enhanced Green Fluorescent Protein. From biobrick BBa_I714891. Proof of concept to see if genes can be imported from bacteria.
 
-
|-
 
-
|eGFP
 
-
|Enhanced Green Fluorescent Protein. From Chin-Sang plasmid pIC691.
 
-
|-
 
-
|mCherry
 
-
|Red Fluorescent Protein. From Chin-Sang plasmid.
 
-
|-
 
-
|YFP
 
-
|Yellow Fluorescent Protein. From Chin-Sang plasmid.
 
-
|-
 
-
|eCFP
 
-
|Enhanced Cyan Fluorescent Protein. From Chin-Sang plasmid.
 
-
|}
 
-
 
-
==Backbones==
 
-
 
-
* Since it is possible to microinject plasmids into ''C. elegans'', we're working on a combination backbone that will produce GFP in both ''E. coli'' and ''C. elegans'', as well as providing antibiotic selection markers for ''E. coli'', and the cutsites from Standard 10.
 
-
* Another project we're pursuing is the possible usage of double-stranded RNA as a liaison for DNA import. If this works, we'll have hybrid strand backbones for that method of transformation as well.
 
-
 
-
=Optogenetics (R.''C. elegans'')=
 
-
 
-
==Promoters==
 
-
 
-
{|
 
-
!Name
 
-
!Description
 
-
!Strength
 
-
|-
 
-
|}
 
-
 
-
==Coding Squences==
 
-
 
-
{|
 
-
!Name
 
-
!Description
 
-
|-
 
-
|ChR2
 
-
|Channelrhodopsin-2. Permits entry of positive ions in response to 460 nm light. Can be used to trigger nerve cells.
 
-
|-
 
-
|NpHR
 
-
|Halorhodopsin. Permits entry of chloride ions in response to 580 nm light. Can be used to block nerve cells from firing.
 
-
|}
 
{{:Team:Queens-Canada/foot}}
{{:Team:Queens-Canada/foot}}

Revision as of 18:23, 26 October 2010

WormWorks Parts List

Show/hide parts as entered in the [http://partsregistry.org Registry].

<groupparts>iGEM010 Queens-Canada</groupparts>