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Team:Wisconsin-Madison/results
From 2010.igem.org
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====Results==== | ====Results==== | ||
| + | [[Image:Cell Survivability Testing Graphs.jpg]] | ||
==Timed Lysis: Inducible/Repressible Promoter== | ==Timed Lysis: Inducible/Repressible Promoter== | ||
| - | ''' | + | {| border="1" cellpadding="5" cellspacing="0" align="center" |
| + | |+ align="center" style="color:#000000;" |''Parts used in these Experiments'' | ||
| + | |- | ||
| + | |'''Part Number''' | ||
| + | |'''Function''' | ||
| + | |'''Induction''' | ||
| + | |'''Zip File''' | ||
| + | |- | ||
| + | |<partinfo>BBa_k318513</partinfo> | ||
| + | |Produces RFP | ||
| + | |stationary phase | ||
| + | |513 | ||
| + | |} | ||
| + | ===Background== | ||
| + | '''gadAp: A pH Sensitive Promoter''' | ||
Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part | Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part | ||
| - | ===pH Induction=== | + | ===pH Induction Results=== |
| - | ===Stationary Phase Induction=== | + | Download procedure [[Media:UW_???.pdf|here]] or continue to experimental protocol page |
| + | |||
| + | ===Stationary Phase Induction Results=== | ||
| + | |||
| + | Download procedure [[Media:UW_???.pdf|here]] or continue to experimental protocol page | ||
<html> | <html> | ||
<div> | <div> | ||
</html> | </html> | ||
Revision as of 17:51, 26 October 2010
Encapsulation
| Part Number | Function | Expression Type | Zip File |
| <partinfo>BBa_k318500</partinfo> | Produces Trascription Factor RcsB | Inducible - IPTG | 500 |
| <partinfo>BBa_k318501</partinfo> | Produces Trascription Factor RcsA | Inducible - IPTG | 501 |
| <partinfo>BBa_k318502</partinfo> | Produces Trascription Factor RcsA & RcsB | Inducible - IPTG | 502 |
| <partinfo>BBa_k200021</partinfo> | Empty Vector/Contol | Inducible - IPTG | NA |
Colonic Acid Quantification
Background
Colonic Acid is a polysaccharide containing a repeat unit with D-glucose, L-fucose, D-galactose, and D-glucuronate. Biological extracts often contain compounds, which under heating with H2SO4 yield brown products absorbing between 396 nm and 427 nm. Colonic acid can be estimated by measuring L-fucose content.
Download procedure here or continue to experimental protocol page
Results
Cell Survivability in Acidic Conditions
Background
Download procedure here or continue to experimental protocol page
Results
Timed Lysis: Inducible/Repressible Promoter
| Part Number | Function | Induction | Zip File |
| <partinfo>BBa_k318513</partinfo> | Produces RFP | stationary phase | 513 |
=Background
gadAp: A pH Sensitive Promoter Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part
pH Induction Results
Download procedure here or continue to experimental protocol page
Stationary Phase Induction Results
Download procedure here or continue to experimental protocol page
