Team:Stockholm/9 June 2010

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(Difference between revisions)
(→Testing DH5α competent cells by transformation)
(→Testing DH5α competent cells by transformation)
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I transformed both my own cells from yesterday and also the kit's competent cells. Each one had a two serial 10-fold dilution.
I transformed both my own cells from yesterday and also the kit's competent cells. Each one had a two serial 10-fold dilution.
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===Preparation LB agar plates with ampicillin resistance===
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LB agar:
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*25 g LB
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*15 g bactoagar
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*Fill up to 1 L with distilled water
 +
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# Autoclave for 120°C, 20 min
 +
# Cool down
 +
# Add 200 mg ampiciliin in 1,8 ml water
 +
# Pour our on plates

Revision as of 21:40, 6 July 2010

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Nina

Testing DH5α competent cells by transformation

This was performed according to tranformation protocol From NEB 5-alpha competent E.coli (High Efficiency) NEW ENGLAND BioLabs.

I transformed both my own cells from yesterday and also the kit's competent cells. Each one had a two serial 10-fold dilution.

Preparation LB agar plates with ampicillin resistance

LB agar:

  • 25 g LB
  • 15 g bactoagar
  • Fill up to 1 L with distilled water
  1. Autoclave for 120°C, 20 min
  2. Cool down
  3. Add 200 mg ampiciliin in 1,8 ml water
  4. Pour our on plates
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