From 2010.igem.org
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| - | Protocol 2: 5mL Overnight
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| - | Reagents:
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| - | * 10mL culture tube Use 16mm x 160mm or 16mm x 125 mm
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| - | * 5mL LB
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| - | * Concentrated antibiotics
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| - | * Single colonies on a plate (Best not to start O/N from glycerol stocks)
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| - | Procedure:
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| - | * Pipet 1.5-20uL of the appropriate antibiotic to the appropriate working concentration into culture tube.
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| - | * Add 5mL non-contaminated LB. Then add antibiotic.
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| - | * Select single colony using a sterile instrument that has been cooled and inoculate the liquid culture.
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| - | * Place culture tube in incubator at 37<sup>o</sup>C overnight shaking vigorously (250 rpm).
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| - | Notes:
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| - | For I0500, culture overnight; then add 1mM ITPG in morning and continue culture for another 3 hours. Then miniprep.
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| - | [[Team:Alberta/Notebook/protocols| Back]]
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Latest revision as of 17:16, 26 October 2010
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