From 2010.igem.org
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| - | Protocol 15: Making LB Plates
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| - | Procedure:
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| - | LB Agar (To Make 1L):
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| - | *Before you start:
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| - | ** Use AGAR not agarose!
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| - | ** Use an Erlenmeyer flask that is twice the volume of your solution (avoid spillage in autoclave).
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| - | ** Keep your flask on a stir plate with a stir rod.
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| - | ** Put Aluminum foil on the top of the flask and put autoclave tape on.
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| - | ** DON`T let it solidify after autoclaving by keeping it on a stir plate while it cools.
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| - | ** DON`T put tubes of antibiotics back into freezer.
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| - | * Add the following to 800 ml MilliQ H20:
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| - | {|
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| - | |Bacto-tryptone || 10g
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| - | |-
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| - | |Yeast extract || 5g
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| - | |-
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| - | |NaCl || 10g
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| - | |}
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| - | * Adjust pH to 7.5 with NaOH (Optional).
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| - | * Add 15g agar.
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| - | * Melt agar into solution in the microwave (Optional).
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| - | * Adjust volume to 1L with MilliQ H20
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| - | * Autoclave on the liquid cycle (no drying time) (aka LIQ20) for 30 minutes.
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| - | * Wait for it to cool to touchable temperature and then add antibiotics if necessary. Amount of antibiotic required should be in the protocol binder.
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| - | * Pour plates, making sure they are color coded with the type of antibiotic that you used.
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| - | Making LB Broth:
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| - | *All of the above WITHOUT AGAR.
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| - | [[Team:Alberta/Notebook/protocols| Back]]
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| - | {{Team:Alberta/endMainContent}}
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Latest revision as of 17:13, 26 October 2010
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