"
Team:Tsinghua/Notebook/31 July 2010
From 2010.igem.org
(Difference between revisions)
Shelly (Talk | contribs)
(New page: == Module I, Group 2c == To solve the problem of severe dimerization of primers, we tried to adjust PCR system and use different concentration of upstream and downstream primers. As the c...)
Newer edit →
(New page: == Module I, Group 2c == To solve the problem of severe dimerization of primers, we tried to adjust PCR system and use different concentration of upstream and downstream primers. As the c...)
Newer edit →
Revision as of 16:43, 26 October 2010
Module I, Group 2c
To solve the problem of severe dimerization of primers, we tried to adjust PCR system and use different concentration of upstream and downstream primers. As the concentration of PCR product is too low to be digested and purification, we attempted to use PCR product got yesterday as the template for PCR today.
PCR system (pyrobest):
H2O 17.3ul 5×buffer 2.5μl dNTP 2.5μl primer1 1μl primer2 0.5μl template 1μl FastPFU 0.2μl Total 20μl
Result: to my disappoint, after gel purification,target fragments disappeared! What's worse, no PCR product of Chlr is seen in the gel photograph actually.
