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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/25
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(New page: ==2010/09/10 Saturday(NEX)== ===Experiment:PCR=== '''Member'''<br/> easily '''Material'''<br/> *sense primers of pSB1C3 *antisense primers of pSB1C3 '''Procedure'''<br/> Refer to <html>...) |
(→Experiment:Agarose gel electrophoresis) |
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Refer to <html><a href="Agarose gel electrophoresis">protocol4</a></html> | Refer to <html><a href="Agarose gel electrophoresis">protocol4</a></html> | ||
| - | '''Result''' | + | '''Result'''<br/> |
[[Image:100925.jpg]]<br/> | [[Image:100925.jpg]]<br/> | ||
pSB1C3 were appeared | pSB1C3 were appeared | ||
| - | |||
===Experiment:DNA purification with silica gel=== | ===Experiment:DNA purification with silica gel=== | ||
Revision as of 15:59, 26 October 2010
Contents |
2010/09/10 Saturday(NEX)
Experiment:PCR
Member
easily
Material
- sense primers of pSB1C3
- antisense primers of pSB1C3
Procedure
Refer to protocol3
Experiment:Ligatiom
Member
easily
Material
- bcsA from E.coli 20μl
- bcsB from E.coli 20μl
- 2×ligation mix 40μl
Procedure
Follow to protocol7
Experiment:Agarose gel electrophoresis
Member
easily
Material
- Today's PCR productions
- Today's ligation productions
- Agarose gel
Procedure
Refer to protocol4
Result
pSB1C3 were appeared
Experiment:DNA purification with silica gel
Member
easily
Materials
- pSB1C3
Procedure
See protocol5-2
Experiment:Ligatiom
Member
easily
Material
- pSB1C3 3μl
- bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
- 2×ligation mix 6μl
Procedure
Follow to protocol7
