Team:Stockholm/5 July 2010

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= Andreas =
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=== Plasmid preparations ===
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Prepared plasmids from ON cultures.
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'''DNA concentrations'''
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{|border="1" cellspacing="0" cellpadding="2"
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|'''Sample'''
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|'''DNA conc (ng/ul)'''
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|'''A(260)/A(280)'''
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|-
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|pSB1A3.BBa_J04450
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|66.8
 +
|n/a
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|-
 +
|pSB1C3.BBa_J04450
 +
|36.5
 +
|n/a
 +
|-
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|pSB1AC3.BBa_J04450
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|41.0
 +
|n/a
 +
|-
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|pSB1AK3.BBa_J04450
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|10.8
 +
|n/a
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|-
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|pMA.BBa_J18930
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|33.3
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|n/a
 +
|-
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|pMA.BBa_J18931
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|52.5
 +
|n/a
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|-
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|pMA.BBa_J18932
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|34.1
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|n/a
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|}
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 +
=== Part and primer design ===
 +
 +
I investigated Mimmi's primers, most specifically the ones designed for ligation of our CPPs (TAT, LMWP and Transportan 10). Me and Johan discussed whether we should order them as is, or if we should instead have the genes synthesized. We decided that we should rethink the matter until tomorrow, when we can have a group meeting and discuss this further.
 +
 +
Johan also discovered that several of our genes contain disallowed BioBrick restriction sites:
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* '''Tyrosinase''' contains one NgoMIV site at 500 bp.
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* '''yCCS''' contains one EcoRI site at 230 bp.
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* '''bFGF''' contains one AgeI site at 350 bp.
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 +
These need to be removed. We will discuss this tomorrow.
= Hassan =
= Hassan =

Revision as of 11:14, 6 July 2010

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Contents


Andreas

Plasmid preparations

Prepared plasmids from ON cultures.

DNA concentrations

Sample DNA conc (ng/ul) A(260)/A(280)
pSB1A3.BBa_J04450 66.8 n/a
pSB1C3.BBa_J04450 36.5 n/a
pSB1AC3.BBa_J04450 41.0 n/a
pSB1AK3.BBa_J04450 10.8 n/a
pMA.BBa_J18930 33.3 n/a
pMA.BBa_J18931 52.5 n/a
pMA.BBa_J18932 34.1 n/a

Part and primer design

I investigated Mimmi's primers, most specifically the ones designed for ligation of our CPPs (TAT, LMWP and Transportan 10). Me and Johan discussed whether we should order them as is, or if we should instead have the genes synthesized. We decided that we should rethink the matter until tomorrow, when we can have a group meeting and discuss this further.

Johan also discovered that several of our genes contain disallowed BioBrick restriction sites:

  • Tyrosinase contains one NgoMIV site at 500 bp.
  • yCCS contains one EcoRI site at 230 bp.
  • bFGF contains one AgeI site at 350 bp.

These need to be removed. We will discuss this tomorrow.

Hassan

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