Team:Michigan/Media Recipes

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*500 mL DI water
*500 mL DI water
''autoclave to sterilize''
''autoclave to sterilize''
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 +
'''LB media with agar'''
 +
*10 g LB broth
 +
*7.5 g agar
 +
*500 mL DI water
 +
''autoclave to sterilize''
 +
 +
''add antibiotics when warm to touch (about 50C)''
 +
 +
''pour when warm to the touch (about 50C)''
'''Kanamycin Antibiotic Stock Solution (50 mg/mL)'''
'''Kanamycin Antibiotic Stock Solution (50 mg/mL)'''

Revision as of 22:31, 5 July 2010


Michigan Header




Media Recipes

LB media

  • 10 g LB broth
  • 500 mL DI water

autoclave to sterilize

LB media with agar

  • 10 g LB broth
  • 7.5 g agar
  • 500 mL DI water

autoclave to sterilize

add antibiotics when warm to touch (about 50C)

pour when warm to the touch (about 50C)

Kanamycin Antibiotic Stock Solution (50 mg/mL)

  • 250 mg of kanamycin
  • 5 mL of DI water

filter sterilize and make 1 mL aliquots to store in the -20C freezer

Tetracycline Antibiotic Stock Solution (50 mg/mL)

  • 250 mg of tetracycline
  • 2.5 mL of DI water
  • 2.5 mL of ethanol

filter sterilize and make 1 mL aliquots to store in the -20C freezer