Team:Michigan/Protocols

From 2010.igem.org

(Difference between revisions)
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[[Media:Protocol_for_DNA_Purification.pdf|DNA Purification]]
[[Media:Protocol_for_DNA_Purification.pdf|DNA Purification]]
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[[Media:Protocol_for_DNA_Purification.pdf|Ligation]]
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[[Media:Ligation_Protocol.pdf|Ligation]]
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*Gingo Bioworks Protocol
 +
*Jeremy Minty Protocol

Revision as of 21:58, 5 July 2010


Michigan Header




Protocols

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Electroporation

Miniprep

DNA Digest

Running a Gel

DNA Purification

Ligation

  • Gingo Bioworks Protocol
  • Jeremy Minty Protocol