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Team:Michigan/Protocols
From 2010.igem.org
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==Protocols== | ==Protocols== | ||
| - | ''Cell Culture'' | + | '''Cell Culture''' |
[[Media:6-28-2010_Protocol_for_making_cultures_from_a_-80C_freezer_stock.pdf|Making cultures from a -80C freezer stock]] | [[Media:6-28-2010_Protocol_for_making_cultures_from_a_-80C_freezer_stock.pdf|Making cultures from a -80C freezer stock]] | ||
| Line 17: | Line 17: | ||
[[Media:6-27-2010_P._putida_antibiotic_resistance_protocol.pdf|''P. putida'' KT2440 antibotic resistance tolerance]] | [[Media:6-27-2010_P._putida_antibiotic_resistance_protocol.pdf|''P. putida'' KT2440 antibotic resistance tolerance]] | ||
| - | ''DNA Manipulation'' | + | '''DNA Manipulation''' |
[[Media:General_Transformation_Protocol.pdf|Transformation]] | [[Media:General_Transformation_Protocol.pdf|Transformation]] | ||
Revision as of 21:26, 5 July 2010
Protocols
Cell Culture
Making cultures from a -80C freezer stock
P. putida KT2440 antibotic resistance tolerance
DNA Manipulation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Electroporation
