Team:Michigan/Protocols

From 2010.igem.org

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==Protocols==
==Protocols==
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Cell Culture
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''Cell Culture''
[[Media:6-28-2010_Protocol_for_making_cultures_from_a_-80C_freezer_stock.pdf|Making cultures from a -80C freezer stock]]
[[Media:6-28-2010_Protocol_for_making_cultures_from_a_-80C_freezer_stock.pdf|Making cultures from a -80C freezer stock]]
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[[Media:6-27-2010_P._putida_antibiotic_resistance_protocol.pdf|''P. putida'' KT2440 antibotic resistance tolerance]]
[[Media:6-27-2010_P._putida_antibiotic_resistance_protocol.pdf|''P. putida'' KT2440 antibotic resistance tolerance]]
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DNA Manipulation
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''DNA Manipulation''
[[Media:General_Transformation_Protocol.pdf|Transformation]]
[[Media:General_Transformation_Protocol.pdf|Transformation]]

Revision as of 21:26, 5 July 2010


Michigan Header




Protocols

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Electroporation

Miniprep