Team:Groningen/Biobricks

From 2010.igem.org

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'''Summary:'''
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'''Summary:'''<br>
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Below is given a short description of all the biobricks in our registry.<br><br>
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Below is given a short description of all the biobricks in our registry.
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'''Chaplins'''<br><br>
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<partinfo>BBa_K305001</partinfo> Hydrophobic protein chaplin C (chpC)<br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.<br>
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'''Chaplins'''
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<partinfo>BBa_K305002</partinfo> chaplin C (chpDC) with modified sortase binding motive<br>
-
 
+
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. <br>
-
<partinfo>BBa_K305001</partinfo> Hydrophobic protein chaplin C (chpC)
+
Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.    <br>
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+
<partinfo>BBa_K305003</partinfo> Hydrophobic protein chaplin E (chpE) <br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
+
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.<br>
-
 
+
<partinfo>BBa_K305004</partinfo> Hydrophobic protein chaplin H (chpH)<br>
-
<partinfo>BBa_K305002</partinfo> chaplin C (chpDC) with modified sortase binding motive
+
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.<br>
-
 
+
<partinfo>BBa_K305005</partinfo> Sortase srtA <br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.  
+
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall<br><br>
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'''Promotors'''<br><br>
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Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.     
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<partinfo>BBa_K305006</partinfo> yqxMpr <br>
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+
yqxM promotor effective in biofilm formation, regulates tasA production<br>
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<partinfo>BBa_K305003</partinfo> Hydrophobic protein chaplin E (chpE)  
+
<partinfo>BBa_K305007</partinfo> srfApr 1<br>
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+
Promotor for the surfactin regulatory gene.<br>
-
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
+
<partinfo>BBa_K305008</partinfo> srfApr 2<br>
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+
Promotor for the surfactin regulatory gene.<br>
-
<partinfo>BBa_K305004</partinfo> Hydrophobic protein chaplin H (chpH)
+
<partinfo>BBa_K305009</partinfo> srfApr1>GFP  <br>
-
 
+
Promotor for the surfactin regulatory gene with GFP. <br>
-
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
+
<partinfo>BBa_K305010</partinfo> srfApr2>GFP<br>
-
 
+
Promotor for the surfactin regulatory gene with GFP. <br><br>
-
<partinfo>BBa_K305005</partinfo> Sortase srtA  
+
'''Plasmid Backbone'''<br><br>
-
 
+
<partinfo>BBa_K305011</partinfo> Subtilin inducible expression plasmid for Bacillus subtilis<br>
-
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
+
-
 
+
-
 
+
-
 
+
-
'''Promotors'''
+
-
 
+
-
<partinfo>BBa_K305006</partinfo> yqxMpr  
+
-
 
+
-
yqxM promotor effective in biofilm formation, regulates tasA production
+
-
 
+
-
<partinfo>BBa_K305007</partinfo> srfApr 1
+
-
 
+
-
Promotor for the surfactin regulatory gene.
+
-
 
+
-
<partinfo>BBa_K305008</partinfo> srfApr 2
+
-
 
+
-
Promotor for the surfactin regulatory gene.
+
-
 
+
-
<partinfo>BBa_K305009</partinfo> srfApr1>GFP   
+
-
 
+
-
Promotor for the surfactin regulatory gene with GFP.
+
-
 
+
-
<partinfo>BBa_K305010</partinfo> srfApr2>GFP
+
-
 
+
-
Promotor for the surfactin regulatory gene with GFP.
+
-
 
+
-
 
+
-
 
+
-
'''Plasmid Backbone'''
+
-
 
+
-
<partinfo>BBa_K305011</partinfo> Subtilin inducible expression plasmid for Bacillus subtilis
+
-
 
+
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was  
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was  
constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.
constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.

Revision as of 10:44, 26 October 2010

Biobricks

Here is our registry of our biobricks: <groupparts>iGEM010 Groningen</groupparts>


Summary:
Below is given a short description of all the biobricks in our registry.

Chaplins

<partinfo>BBa_K305001</partinfo> Hydrophobic protein chaplin C (chpC)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
<partinfo>BBa_K305002</partinfo> chaplin C (chpDC) with modified sortase binding motive
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.
<partinfo>BBa_K305003</partinfo> Hydrophobic protein chaplin E (chpE)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
<partinfo>BBa_K305004</partinfo> Hydrophobic protein chaplin H (chpH)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
<partinfo>BBa_K305005</partinfo> Sortase srtA
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall

Promotors

<partinfo>BBa_K305006</partinfo> yqxMpr
yqxM promotor effective in biofilm formation, regulates tasA production
<partinfo>BBa_K305007</partinfo> srfApr 1
Promotor for the surfactin regulatory gene.
<partinfo>BBa_K305008</partinfo> srfApr 2
Promotor for the surfactin regulatory gene.
<partinfo>BBa_K305009</partinfo> srfApr1>GFP
Promotor for the surfactin regulatory gene with GFP.
<partinfo>BBa_K305010</partinfo> srfApr2>GFP
Promotor for the surfactin regulatory gene with GFP.

Plasmid Backbone

<partinfo>BBa_K305011</partinfo> Subtilin inducible expression plasmid for Bacillus subtilis
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.

Retrieved from "http://2010.igem.org/Team:Groningen/Biobricks"