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Latest revision as of 10:31, 26 October 2010

Contents 1 Andreas 1.1 Testing Top10 competent cells and antibiotic agar plates 1.2 Plasmid preparation of pEX vector 1.3 Transformation with pMA vector 2 Hassan

Andreas

Testing Top10 competent cells and antibiotic agar plates

Results from 22/6

Transformed plasmid	Plate	Growth
pEX	100 ug/ml Amp	Yes
None	100 ug/ml Amp	No
pEX	25 ug/ml Cm	No
None	25 ug/ml Cm	No

Growth on Amp plate indicated transformation competence. No growth on other plates indicated good antibiotic selection.

Note: The transformed pEX vector was thought to carry a Cm resistance cassette insert. This was, however, discovered to be wrong.

Plasmid preparation of pEX vector

Two clones picked and each inoculated in 5 ml LB with Amp and grown ON in 37°C, 250 rpm.

Transformation with pMA vector

The pMA (BBa_K157000) vector could become useful for cloning and construction of our parts, as it carries the complete Freiburg prefix and suffix sequences (in contrast to the standard BioBrick vectors pSB1x3).

• Transformed Top10 cells with pMA vector carrying a Freiburg fusion His tag (BBa_K157011) insert. Cells plated on 100 ug/ml Amp plates.

Hassan

Started to use softwares for making 3D animations and/or movies, started by making protein A and IgG Protease.

softwares used:

1. Molecular Maya
2. Chimera