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SDU-Denmark/5 July 2010
From 2010.igem.org
(New page: Phototaxis Progress report:<br><br> ''BioBrick Design:'' We designed and ordered the following primers:<br> // Melting points calculated: http://www.basic.northwestern.edu/biotools/oligoc...)
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Revision as of 13:55, 5 July 2010
Phototaxis
Progress report:
BioBrick Design: We designed and ordered the following primers:
// Melting points calculated: http://www.basic.northwestern.edu/biotools/oligocalc.html (Oligocalc)
NpSRII-HtrII-EcTsr primers:
Upstream primer:
5' - GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGTGGGACTTACG - 3' (Melting point basic: 71°C)
Downstream primer:
5' - GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTAAAATGTTTCCCAGTTCTCCTCG - 3' (Melting point basic: 71°C)
ninaB primers (Retinal enzym):
Upstream primer:
5' - GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGCAGCCGGTGTCTTCAA - 3' (Melting point basic: 73°C)
Downstream primer:
5' - GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTAAATGGCATTGGGTGCAA - 3' (Melting point basic: 71°C)
References:
Flagella
Biobrick design:
FlhDC mutationsprimers:
Passer til komplimentærstrengen:
5' - GGCAGCTTTGCCCGCAGCTTATGTC - 3' (Melting point basic: 63°C)
Passer til coding strand:
5' - GACATAAGCTGCGGGCAAAGCTGCC - 3' (Melting point basic: 63°C)
Modelling
Progress report:
Working hypothesise:
