Team:TU Delft/protocols/ligation
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(New page: ==Ligation== Ligations (pasting plasmid DNA) were performed at the appropriate temperature with the appropriate buffer in the appropriate concentration, according to the supplier. React...)
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(New page: ==Ligation== Ligations (pasting plasmid DNA) were performed at the appropriate temperature with the appropriate buffer in the appropriate concentration, according to the supplier. React...)
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Revision as of 13:17, 5 July 2010
Ligation
Ligations (pasting plasmid DNA) were performed at the appropriate temperature with the appropriate buffer in the appropriate concentration, according to the supplier.
Reaction for one sample:
DNA insert | x μL |
DNA vector | x μL (for 1×) |
T4 Ligation buffer (10×) | 2.5 μL (for 1×) |
T4 Ligase | x μL |
H2O | x μL |
25,0 μL (final contration ~100 ng/μL) |
Transform circa half of the ligation mix. Incubate at 16 °C o/n