Team:TU Delft/protocols/ligation

From 2010.igem.org

(Difference between revisions)
Ebrinkman (Talk | contribs)
(New page: ==Ligation== Ligations (pasting plasmid DNA) were performed at the appropriate temperature with the appropriate buffer in the appropriate concentration, according to the supplier. React...)
Newer edit →

Revision as of 13:17, 5 July 2010

Ligation

Ligations (pasting plasmid DNA) were performed at the appropriate temperature with the appropriate buffer in the appropriate concentration, according to the supplier.


Reaction for one sample:

DNA insert x μL
DNA vector x μL (for 1×)
T4 Ligation buffer (10×) 2.5 μL (for 1×)
T4 Ligase x μL
H2O x μL
25,0 μL (final contration ~100 ng/μL)


Transform circa half of the ligation mix. Incubate at 16 °C o/n