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Team:TU Delft/23 June 2010 content
From 2010.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following: | Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following: | ||
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | ||
| - | | | + | |Componet |
| - | | | + | |Sample |
|- | |- | ||
|I10341 | |I10341 | ||
| - | |3 | + | |3 μL |
|- | |- | ||
|J611XX | |J611XX | ||
| - | |4 | + | |4 μL |
|- | |- | ||
|pSB3C5 | |pSB3C5 | ||
| - | |1.5 | + | |1.5 μL |
|- | |- | ||
|Ligation Buffer | |Ligation Buffer | ||
| - | |3 | + | |3 μL |
|- | |- | ||
|T4 ligase | |T4 ligase | ||
| - | |1 | + | |1 μL |
|- | |- | ||
|H20 | |H20 | ||
| - | |15 | + | |15 μL |
|} | |} | ||
| + | |||
| + | |||
We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. | We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. | ||
The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees. | The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees. | ||
Revision as of 11:49, 5 July 2010
LAB WORK
Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following:
| Componet | Sample |
| I10341 | 3 μL |
| J611XX | 4 μL |
| pSB3C5 | 1.5 μL |
| Ligation Buffer | 3 μL |
| T4 ligase | 1 μL |
| H20 | 15 μL |
We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.
