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Team:TU Delft/23 June 2010 content
From 2010.igem.org
(Difference between revisions)
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Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following: | Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following: | ||
| - | {| style="color: | + | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" |
|'''-''' | |'''-''' | ||
| - | |''' | + | |'''μL''' |
|- | |- | ||
|I10341 | |I10341 | ||
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|15 | |15 | ||
|} | |} | ||
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We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. | We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. | ||
The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees. | The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees. | ||
Revision as of 11:46, 5 July 2010
LAB WORK
Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following:
| - | μL |
| I10341 | 3 |
| J611XX | 4 |
| pSB3C5 | 1.5 |
| Ligation Buffer | 3 |
| T4 ligase | 1 unit |
| H20 | 15 |
We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.
