Team:UNIPV-Pavia/Calendar/June/settimana4
From 2010.igem.org
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(→June, 23rd) |
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After MiniPrep, purified DNA was quantified with NanoDrop. | After MiniPrep, purified DNA was quantified with NanoDrop. | ||
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| I6 || 193 ng/ul | | I6 || 193 ng/ul | ||
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Digestion of: | Digestion of: | ||
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| ''Culture'' || ''Kind'' || ''Final reaction volume (ul) '' || ''DNA (ul)'' || ''H20 (ul)'' || ''Enzyme 1'' || ''Enzyme 2'' || ''Buffer H'' | | ''Culture'' || ''Kind'' || ''Final reaction volume (ul) '' || ''DNA (ul)'' || ''H20 (ul)'' || ''Enzyme 1'' || ''Enzyme 2'' || ''Buffer H'' | ||
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{| border='1' align='center' | {| border='1' align='center' | ||
- | || '''Part | + | || '''Part''' || '''RFP (a.u.)''' |
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||<partinfo>BBa_J23118</partinfo>||1429 | ||<partinfo>BBa_J23118</partinfo>||1429 | ||
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||<partinfo>BBa_J23110</partinfo>|| 844 | ||<partinfo>BBa_J23110</partinfo>|| 844 | ||
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||<partinfo>BBa_J23114</partinfo>|| 256 | ||<partinfo>BBa_J23114</partinfo>|| 256 | ||
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||<partinfo>BBa_J23116</partinfo>|| 396 | ||<partinfo>BBa_J23116</partinfo>|| 396 | ||
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|} | |} | ||
Soon quantitative testes will be performed to quantify each promoter's strength in terms of RPU. | Soon quantitative testes will be performed to quantify each promoter's strength in terms of RPU. |
Revision as of 08:53, 5 July 2010
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Digestion of:
Digestions were incubated at 37°C for 3 hours, gel run and gel-extracted. Ligations were performed ON at 16°C:
These four promoters from Andersone Promoters Collection were choosed for their strength, measured in Arbitrary Units:
Soon quantitative testes will be performed to quantify each promoter's strength in terms of RPU. June, 24thJune, 25th
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