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	= Week #5=		= Week #5=

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Revision as of 04:20, 26 October 2010

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Contents 1 Week #5 2 04th October - 08 October 2010 2.1 Monday 2.2 Tuesday 2.3 Wensday

Week #5

04th October - 08 October 2010

Monday

• Let's picked up colonys our transformant cells and prepare all to do miniprep.

For this we used 30ml de LB liquid agar medium per falcon tube at 80μ/ml

Cloramphenicol concentration we left overnight culture to 37º

Tuesday

With a overnight inoculum we did a miniprep.

The miniprep was done with a Quiagen Kit.

Then we going to do a duble digest with EcoRI and PstI to check out our ligations.

DNA	5μl
Buffer NB2	1μl
EcoRI	0.5μl
PstI	0.5μl
H2O	2.7μl
BSA	0.3μl
Total	10μl

Wensday

We run a gel to confirm our ligations as follow

• PCR 1 Lig S with Psb1C3 Confirmed
• PCR 2 Lig S with Psb1C3 Non confirmed
• PCR 3 Lig S with Psb1C3 Confirmed
• PCR 4 Lig C with Psb1C3 Non confirmed