Team:Michigan/Oil Sands October
From 2010.igem.org
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PCR Screening: | PCR Screening: | ||
- | 8 colonies were picked from each transformation plate except for VP130 and suspended in 50 uL of water on a microplate. | + | 8 colonies were picked from each transformation plate except for VP130 and suspended in 50 uL of water on a microplate.: |
*Plate Layout | *Plate Layout | ||
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The first two rows (Flu 1:1 and 1:3) were PCR'd by adding 1 uL of each sample to a PCR tube with a drop of mineral oil in them. A master mix was made and 50 uL was added to each tube (should have been 49 uL). | The first two rows (Flu 1:1 and 1:3) were PCR'd by adding 1 uL of each sample to a PCR tube with a drop of mineral oil in them. A master mix was made and 50 uL was added to each tube (should have been 49 uL). | ||
- | Master Mix (18x) | + | Master Mix (18x): |
*180 uL 5x Phusion Buffer | *180 uL 5x Phusion Buffer | ||
*18 uL 10 mM dNTPs | *18 uL 10 mM dNTPs | ||
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*585 uL Ultra pure H2O | *585 uL Ultra pure H2O | ||
- | PCR Program | + | PCR Program: |
*1. 95 C for 10 min | *1. 95 C for 10 min | ||
*2. 95 C for 30 sec | *2. 95 C for 30 sec |
Revision as of 04:11, 26 October 2010