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Team:Stockholm/25 October 2010
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Revision as of 00:13, 26 October 2010
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Mimmi
SOD activity
- continuing
| mix | samples | blank 1 | blank 3 |
|---|---|---|---|
| sample solution | 20 | ||
| ddH2O | 20 | 20 | |
| WST solution | 200 | 200 | 200 |
| Enzyme solution | 20 | 20 | |
| dilution buffer | 20 | ||
| tot | 240µl | 240µl | 240µl |
- Incubate in 37°C for 20 min
- Measure A440 with nanodrop
- SOD activity (inhibition %) = ((Ablank1 - Ablank3) - (Asample - Ablank2))/(Ablank1 - Ablank3) x 100
