Team:Stockholm/17 October 2010

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Revision as of 23:54, 25 October 2010

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Contents

Mimmi

clone non-CPP operon

colony PCR

mix x12 primers samples
mastermix 24.5 pEX_VF pEX.SOD.RBS.yCCS 1-4
DNA 0.5 pEX_VR pEX.SOD.his.RBS.yCCS 1-4
tot 25µl pEX.his.SOD.RBS.yCCS 1-4
positive control (pSB1C3.LMWP.SOD.his)

Gel

Place for picture.jpg
well sample well sample
1 ladder 12 ladder
2 pEX.S_yC 1 13 pEX.hS_yC 1
3 pEX.S_yC 2 14 pEX.hS_yC 2
4 pEX.S_yC 3 15 pEX.hS_yC 3
5 pEX.S_yC 4 16 pEX.hS_yC 4
6 pEX.Sh_yC 1
7 pC.Sh_yC 2
8 pC.Sh_yC 3
9 pC.Sh_yC 4



new colony PCR

mix x7 primers samples
mastermix 24.5 pEX_VF pEX.SOD.his.RBS.yCCS 1-7
DNA 0.5 pEX_VR
tot 25µl


ON cultures

  • pEX.SOD.RBS.yCCS 4
  • pEX.his.SOD.RBS.yCCS 3
  • pEX.SOD
  • pEX.yCCS
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