Team:Stockholm/8 June 2010

From 2010.igem.org

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= Johan =
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== Continuation of making competent cells ==
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Step 2-12 in "800 ml of competent cells"
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1 L instead of 800 ml -> 25 st 50 ml Falcon Tubes with 40 ml, 1 st with 15-20 ml
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10) 16/23 = 0,69 ml
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3) Abs:
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Only one bottle
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* 0,029 9:25
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* 0,053 ~1h5min
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* 0,188 ~1h5min
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* 0,316 ~30min
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Both bottles
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* 0,519 ~30min
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* 0,503
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* 0,578 ~7min
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* 0,566
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* 0,598 ~5min = 13:05
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* 0,600
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12) ~160 epps, 100 µl in each.
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They were then all put in the freezer, in two cardboard boxes (no metal blocks used)

Revision as of 23:14, 25 October 2010

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Contents

Nina

Preparation of competent DH5α Escherichia coli cells

Continued from 21/6.

Prepared competent DH5α cells according to the protocol of Morten Nørholm at the Department of Biochemistry & Biophysics Stockholm University.

Absorbance:

  • 1 ml sample from one bottle:
Time OD
30min 0,029
1h 0,053
1h 0,188
30min 0,316
  • 1 ml sample from two bottles:
Time OD
30min 0,519/0,503
7min 0,578/0,566
5min 0,598/0,600


Johan

Continuation of making competent cells

Step 2-12 in "800 ml of competent cells"

1 L instead of 800 ml -> 25 st 50 ml Falcon Tubes with 40 ml, 1 st with 15-20 ml

10) 16/23 = 0,69 ml

3) Abs:

Only one bottle

  • 0,029 9:25
  • 0,053 ~1h5min
  • 0,188 ~1h5min
  • 0,316 ~30min

Both bottles

  • 0,519 ~30min
  • 0,503
  • 0,578 ~7min
  • 0,566
  • 0,598 ~5min = 13:05
  • 0,600

12) ~160 epps, 100 µl in each.

They were then all put in the freezer, in two cardboard boxes (no metal blocks used)

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