Team:TU Delft/Project/sensing/results

From 2010.igem.org

(Difference between revisions)
(→Sensing of Crp)
(→Sensing Results)
Line 5: Line 5:
===pCaiF strength===
===pCaiF strength===
 +
====BBa_K398326====
 +
pCaiF is one of our Biobricks of the sensing project. It's function is to express proteins under glucose limitation conditions. In order to measure its strength we attached the GFP generator in front of it.
 +
Different media, and carbon source concentrations were tested in order to obtain an insight about the protein production during starvation.
 +
 
 +
[[Image:TU_Delft_pCaiF_comp.jpg]]
 +
 +
Also, it is well known that this promotor it's active during diauxic shifts, that's why we measured the GFP production using glucose as a first carbon source (concentration of 1g/L) and Potassium Laurate (5mM) as a secondary carbon source. The results that we got suggest that the part works like a charm! During glucose starvation high levels of cAMP can be found in cells, which enhances the expression of the proteins downstream of this promoter. On the other hand when the secondary carbon source is being catabolized by the cells, cAMP levels drop and thus the expression of genes regulated by pCaiF.
 +
 +
 +
[[Image:TU_Delft_pCaiF_diauxic.jpg]]
 +
 +
View [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398326 BBa_K398326 in the '''parts registry''']
 +
 +
'''Specified Components'''
 +
<partinfo>K398326 SpecifiedComponents</partinfo>
 +
 +
'''Designer:''' <partinfo>K398326 Designer</partinfo>
 +
 +
'''Status:''' <partinfo>K398326 Status</partinfo>
 +
<html><span style="clear:both;display:block;width:100%;"></span></html>
 +
 +
View [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398331 BBa_K398331 in the '''parts registry''']
 +
 +
'''Specified Components'''
 +
<partinfo>K398331 SpecifiedComponents</partinfo>
 +
 +
'''Designer:''' <partinfo>K398331 Designer</partinfo>
 +
 +
'''Status:''' <partinfo>K398331 Status</partinfo>
 +
<html><span style="clear:both;display:block;width:100%;"></span></html>
-
Results & Conclusions
 
<html><center><img src="https://static.igem.org/mediawiki/2010/0/00/TU_Delft_project_navigation.jpg" usemap="#projectnavigation" border="0" /></center><map id="projectnavigation" name="projectnavigation"><area shape="rect" alt="Characterization" title="" coords="309,3,591,45" href="https://2010.igem.org/Team:TU_Delft#page=Project/sensing/characterization" target="" /><area shape="rect" alt="Results" title="" coords="609,3,891,44" href="https://2010.igem.org/Team:TU_Delft#page=Project/sensing/results" target="" /><area shape="rect" alt="Parts" title="" coords="9,3,290,44" href="https://2010.igem.org/Team:TU_Delft#page=Project/sensing/parts" target="" /></map></html>
<html><center><img src="https://static.igem.org/mediawiki/2010/0/00/TU_Delft_project_navigation.jpg" usemap="#projectnavigation" border="0" /></center><map id="projectnavigation" name="projectnavigation"><area shape="rect" alt="Characterization" title="" coords="309,3,591,45" href="https://2010.igem.org/Team:TU_Delft#page=Project/sensing/characterization" target="" /><area shape="rect" alt="Results" title="" coords="609,3,891,44" href="https://2010.igem.org/Team:TU_Delft#page=Project/sensing/results" target="" /><area shape="rect" alt="Parts" title="" coords="9,3,290,44" href="https://2010.igem.org/Team:TU_Delft#page=Project/sensing/parts" target="" /></map></html>

Revision as of 21:38, 25 October 2010

CharacterizationResultsParts

Sensing Results

pCaiF strength

BBa_K398326

pCaiF is one of our Biobricks of the sensing project. It's function is to express proteins under glucose limitation conditions. In order to measure its strength we attached the GFP generator in front of it. Different media, and carbon source concentrations were tested in order to obtain an insight about the protein production during starvation.

TU Delft pCaiF comp.jpg

Also, it is well known that this promotor it's active during diauxic shifts, that's why we measured the GFP production using glucose as a first carbon source (concentration of 1g/L) and Potassium Laurate (5mM) as a secondary carbon source. The results that we got suggest that the part works like a charm! During glucose starvation high levels of cAMP can be found in cells, which enhances the expression of the proteins downstream of this promoter. On the other hand when the secondary carbon source is being catabolized by the cells, cAMP levels drop and thus the expression of genes regulated by pCaiF.


TU Delft pCaiF diauxic.jpg

View [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398326 BBa_K398326 in the parts registry]

Specified Components <partinfo>K398326 SpecifiedComponents</partinfo>

Designer: <partinfo>K398326 Designer</partinfo>

Status: <partinfo>K398326 Status</partinfo>

View [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398331 BBa_K398331 in the parts registry]

Specified Components <partinfo>K398331 SpecifiedComponents</partinfo>

Designer: <partinfo>K398331 Designer</partinfo>

Status: <partinfo>K398331 Status</partinfo>



CharacterizationResultsParts

Retrieved from "http://2010.igem.org/Team:TU_Delft/Project/sensing/results"