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Team:Heidelberg/Notebook/Material
From 2010.igem.org
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=Materials= | =Materials= | ||
== Kits == | == Kits == | ||
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! Description || Name || Firm | ! Description || Name || Firm | ||
| Table Centrifuge || Microfuge'''®''' 18 Centrifuge || Beckman Coulter<sup>TM</sup> | | Table Centrifuge || Microfuge'''®''' 18 Centrifuge || Beckman Coulter<sup>TM</sup> | ||
| + | |- | ||
| Table Centrifuge || MicrofugeR 22'''®''' Centrifuge || Beckman Coulter<sup>TM</sup> | | Table Centrifuge || MicrofugeR 22'''®''' Centrifuge || Beckman Coulter<sup>TM</sup> | ||
| + | |- | ||
| Centrifuge || Allegra X-12 || Beckman Coulter<sup>TM</sup> | | Centrifuge || Allegra X-12 || Beckman Coulter<sup>TM</sup> | ||
| + | |- | ||
| Shaker || VORTEXGENIER 2 || Scientific Industries, SI<sup>TM</sup> | | Shaker || VORTEXGENIER 2 || Scientific Industries, SI<sup>TM</sup> | ||
| + | |- | ||
| Heating plate (magnet stirrer) || MR Hei-Standard || Heidolph | | Heating plate (magnet stirrer) || MR Hei-Standard || Heidolph | ||
| + | |- | ||
| Heatblock || QBD4 || Grant | | Heatblock || QBD4 || Grant | ||
| + | |- | ||
| Heatblock (shakeing function) || Thermomixer comfort || Eppendorf | | Heatblock (shakeing function) || Thermomixer comfort || Eppendorf | ||
| - | |- | + | |- |
| PCR-machine || MyCycler<sup>TM</sup> thermo cycler || BioRad | | PCR-machine || MyCycler<sup>TM</sup> thermo cycler || BioRad | ||
| + | |- | ||
| UV-chamber || Transluminator || Vilber Lourmat | | UV-chamber || Transluminator || Vilber Lourmat | ||
| + | |- | ||
| Scale (fine) || Pioneer<sup>TM</sup> PA114C || OHAUS | | Scale (fine) || Pioneer<sup>TM</sup> PA114C || OHAUS | ||
| + | |- | ||
| Scale || Pioneer<sup>TM</sup> PA4101C || OHAUS | | Scale || Pioneer<sup>TM</sup> PA4101C || OHAUS | ||
| + | |- | ||
| Fridge || KTP 1750 Premium || Liebherr | | Fridge || KTP 1750 Premium || Liebherr | ||
| + | |- | ||
| Freezer || GP 1366 Premium || Liebherr | | Freezer || GP 1366 Premium || Liebherr | ||
| + | |- | ||
| -86 Freezer || -86°C Ultralow Freezer || NUAIRE<sup>TM</sup> | | -86 Freezer || -86°C Ultralow Freezer || NUAIRE<sup>TM</sup> | ||
| + | |- | ||
| Hood || Tischabzug || Wesemann'''®''' Laboreinrichtung | | Hood || Tischabzug || Wesemann'''®''' Laboreinrichtung | ||
| + | |- | ||
| Draw-off pump || Vacuhand control || Vacubrand | | Draw-off pump || Vacuhand control || Vacubrand | ||
| + | |- | ||
| Incubator || HT Multitron Version 2 || INFORS | | Incubator || HT Multitron Version 2 || INFORS | ||
| + | |- | ||
| Incubator (cell culture) || HERAcell 150 || Thermo electron coorperation | | Incubator (cell culture) || HERAcell 150 || Thermo electron coorperation | ||
| + | |- | ||
| Hood (cell culture) || HERAsafe || Thermo electron coorperation | | Hood (cell culture) || HERAsafe || Thermo electron coorperation | ||
| + | |- | ||
| Plate reader || Infinite M200 || TECAN | | Plate reader || Infinite M200 || TECAN | ||
| + | |- | ||
| FACS || Cytomics FC 500 MPL || Beckman Coulter<sup>TM</sup> | | FACS || Cytomics FC 500 MPL || Beckman Coulter<sup>TM</sup> | ||
| + | |- | ||
| Microscope || CKX41 || Olympus | | Microscope || CKX41 || Olympus | ||
| + | |- | ||
| Computer || || Sun Microsystems | | Computer || || Sun Microsystems | ||
| + | |- | ||
| UV/Visivle Spectrometer || Ultrospec 3300 pro || Amersham Biosciences | | UV/Visivle Spectrometer || Ultrospec 3300 pro || Amersham Biosciences | ||
| + | |- | ||
| Photometer || NanoDrop'''®''' ND-1000 Spectrophotometer || peQLab Biotechnologie GmbH | | Photometer || NanoDrop'''®''' ND-1000 Spectrophotometer || peQLab Biotechnologie GmbH | ||
| + | |- | ||
| Photometer || NanoVue<sup>TM</sup> || General Electric | | Photometer || NanoVue<sup>TM</sup> || General Electric | ||
| + | |- | ||
| Ice Machine || MF22 || SCOTSMAN'''®''' | | Ice Machine || MF22 || SCOTSMAN'''®''' | ||
| + | |- | ||
| Gel electrophoresis chamber || Mupid'''®'''-One || Advance | | Gel electrophoresis chamber || Mupid'''®'''-One || Advance | ||
| + | |- | ||
| pipetting robot || QIAcube<sup>TM</sup> || QIAGEN | | pipetting robot || QIAcube<sup>TM</sup> || QIAGEN | ||
| - | + | |- | |
| + | | Wide field microscope || Nikon Eclipse 90i || Nikon | ||
| + | |- | ||
| PCR machine || MyCycler thermal cycler || BIO-RAD | | PCR machine || MyCycler thermal cycler || BIO-RAD | ||
| + | |- | ||
| Real-time PCR machine || StepOnePlus<sup>TM</sup> Real-time PCR System || Applied Biosystems<sup>TM</sup> | | Real-time PCR machine || StepOnePlus<sup>TM</sup> Real-time PCR System || Applied Biosystems<sup>TM</sup> | ||
| - | |- | + | |- |
|} | |} | ||
Revision as of 21:35, 25 October 2010
Materials
Kits
| Kits | Supplier | Catalog Number |
| HiSpeed® Plasmid Maxi Kit (25) | QIAGEN | 12663 |
| CompactPrep Plasmid Maxi Kit (25) | QIAGEN | 12763 |
| RNeasy® Mini Kit (25) | QIAGEN | 74106 |
| QIAquick® Miniprep Kit (250) | QIAGEN | 27106 |
| QIAquick® Gel Extraction Kit (250) | QIAGEN | 28706 |
| QIAquick® Nucleotid Removal Kit (250) | QIAGEN | 28306 |
| QIAquick® PCR Purification Kit (250) | QIAGEN | 28106 |
| GeneMorph® II EZC Clone Domain Mutagenesis Kit | Stratagene | 200552-5 |
Marker
| Name | Supplier | Range |
| GeneRuler™ High Range DNA Ladder | Fermentas | 10,171-48,502 bp |
| GeneRuler™ 1 kb Plus DNA Ladder | Fermentas | 75-20,000 bp |
Enzymes
| Name | Number/ID | NEB buffer1 | NEB buffer2 | NEB buffer3 | NEB buffer4 | BSA |
| AflII | 27 | 50% | 100% | 25% | 100% | x |
| AgeI | 1 | 100% | 50% | 10% | 75% | |
| ApaI | - | 25% | 50% | 0% | 100% | x |
| AseI | not | 75% | 100% | not | ||
| AvrII | - | 100% | 100% | 50% | 100% | |
| BamHI | 2 | 75% | 100% | 100% | 100% | x |
| BamHI | - | 75% | 100% | 100% | 100% | x |
| BclI | - | 50% | 100% | 100% | 75% | |
| BglI | - | 50% | 75% | 100% | 50% | |
| BglII | 13 | 10% | 75% | 100% | 10% | |
| BsrGI | - | 25% | 100% | 10% | 100% | x |
| DpnI | 6 | 100% | 100% | 75% | 100% | |
| DpnI | - | 100% | 100% | 75% | 100% | |
| EcoRI | - | 100% | 100% | 100% | 100% | |
| EcoRV | - | 50% | 75% | 100% | 50% | x |
| FseI | - | 100% | 75% | 0% | 100% | |
| HindIII | - | 10 50% | 100% | 10% | 50% | |
| HindIII | - | 50% | 100% | 10% | 50% | |
| KpnI | 11 | 100% | 75% | 0% | 50% | x |
| MfeI-HF™ | - | 75% | 50% | 10% | 100% | |
| NarI | 26 | 100% | 75% | 75% | 100% | |
| NcoI | 12 | 100% | 100% | 100% | 100% | |
| NdeI | - | 75% | 100% | 75% | 100% | |
| NheI | - | 100% | 100% | 10% | 100% | x |
| NotI | 14 | 0% | 50% | 100% | 25% | |
| NsiI | - | 10% | 75% | 100% | 25% | |
| PciI | - | 50% | 75% | 100% | 50% | x |
| PstI | 15 | 75% | 75% | 100% | 50% | |
| SacI | 16 | 100% | 50% | 10% | 100% | |
| ScaI | 18 | not | not | 100% | not | |
| SfcI | 19 | 75% | 50% | 10% | 100% | x |
| SfcI | - | 75% | 50% | 10% | 100% | x |
| SpeI | - | 75% | 100% | 25% | 100% | x |
| SphI | - | 100% | 100% | 50% | 100% | |
| SspI | - | 50% | 100% | 50% | 50% | |
| XbaI | 23 | 0% | 100% | 75% | 100% | x |
| XbaI | 56 | 0% | 100% | 75% | 100% | x |
| XhoI | 24 | 75% | 100% | 100% | 100% | x |
| XmaI | 25 | 25% | 50% | 0% | 100% | x |
NEBuffers are color-coded (NEBuffer 1-yellow, NEBuffer 2-blue, NEBuffer 3-red, NEBuffer 4-green) and supplied as 10X
Bacteria
| Name | Purpose | Reference |
| DH5α | Amplification of Plasmids | Invitrogen |
| SCS110 | Amplification of unmethylated Plasmids | Stratagene |
| Top10 | Amplification of Plasmids | Invitrogen |
Media and Antibiotics
- LB (10 g tryptone, 5 g yeast extract, 10 g sodium chloride, ad 1 L ddH2O)
| Antibiotic | Final Concentration | Solvent |
|---|---|---|
| Ampicillin | 100 µg/ml | ddH2O |
| Chloramphenicol | 25 µg/ml | 100% Ethanol |
| Kanamycin | 50 µg/ml | ddH2O |
Primers & Oligos
Please find our Primers & Oligos on an extra subpage.
Instruments
The following is a list of all used Instruments.
| Description | Name | Firm | Table Centrifuge | Microfuge® 18 Centrifuge | Beckman CoulterTM |
|---|---|---|---|---|---|
| Table Centrifuge | MicrofugeR 22® Centrifuge | Beckman CoulterTM | |||
| Centrifuge | Allegra X-12 | Beckman CoulterTM | |||
| Shaker | VORTEXGENIER 2 | Scientific Industries, SITM | |||
| Heating plate (magnet stirrer) | MR Hei-Standard | Heidolph | |||
| Heatblock | QBD4 | Grant | |||
| Heatblock (shakeing function) | Thermomixer comfort | Eppendorf | |||
| PCR-machine | MyCyclerTM thermo cycler | BioRad | |||
| UV-chamber | Transluminator | Vilber Lourmat | |||
| Scale (fine) | PioneerTM PA114C | OHAUS | |||
| Scale | PioneerTM PA4101C | OHAUS | |||
| Fridge | KTP 1750 Premium | Liebherr | |||
| Freezer | GP 1366 Premium | Liebherr | |||
| -86 Freezer | -86°C Ultralow Freezer | NUAIRETM | |||
| Hood | Tischabzug | Wesemann® Laboreinrichtung | |||
| Draw-off pump | Vacuhand control | Vacubrand | |||
| Incubator | HT Multitron Version 2 | INFORS | |||
| Incubator (cell culture) | HERAcell 150 | Thermo electron coorperation | |||
| Hood (cell culture) | HERAsafe | Thermo electron coorperation | |||
| Plate reader | Infinite M200 | TECAN | |||
| FACS | Cytomics FC 500 MPL | Beckman CoulterTM | |||
| Microscope | CKX41 | Olympus | |||
| Computer | Sun Microsystems | ||||
| UV/Visivle Spectrometer | Ultrospec 3300 pro | Amersham Biosciences | |||
| Photometer | NanoDrop® ND-1000 Spectrophotometer | peQLab Biotechnologie GmbH | |||
| Photometer | NanoVueTM | General Electric | |||
| Ice Machine | MF22 | SCOTSMAN® | |||
| Gel electrophoresis chamber | Mupid®-One | Advance | |||
| pipetting robot | QIAcubeTM | QIAGEN | |||
| Wide field microscope | Nikon Eclipse 90i | Nikon | |||
| PCR machine | MyCycler thermal cycler | BIO-RAD | |||
| Real-time PCR machine | StepOnePlusTM Real-time PCR System | Applied BiosystemsTM |
|
