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Team:Michigan/Pili Expression
From 2010.igem.org
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|[[Team:Michigan/Pili_Expression#6/29/2010|6/29/2010]] | |[[Team:Michigan/Pili_Expression#6/29/2010|6/29/2010]] | ||
|[[Team:Michigan/Pili_Expression#6/30/2010|6/30/2010]] | |[[Team:Michigan/Pili_Expression#6/30/2010|6/30/2010]] | ||
| - | | | + | |[[Team:Michigan/Pili_Expression#7/1/2010|7/1/2010]] |
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| - | | - | ||
| Line 45: | Line 45: | ||
*1 mL located in the iGEM box in the Lin lab. | *1 mL located in the iGEM box in the Lin lab. | ||
*1 mL located in the lab freezer. | *1 mL located in the lab freezer. | ||
| + | |||
| + | ==7/1/2010== | ||
| + | Cryopreserved DH5alpha according to protocol procedure on 6/30/2010 | ||
| + | *Put 1 stock in -20C fridge in 1239 | ||
| + | *Put the other stock in the -80C fridge in the Lin lab | ||
Revision as of 17:08, 2 July 2010
| Sunday | Monday | Tuesday | Wednesday | Thursday | Friday | Saturday | |
| Week 1 | - | 6/28/2010 | 6/29/2010 | 6/30/2010 | 7/1/2010 | - | - |
6/28/2010
Made a 500 mL batch of LB broth
- When pouring in distilled water, pour a few mL at a time to avoid clumping.
- It takes 20g of powder to make 1 L of broth
Sterilized broth using the autoclave
- The temperature setting on the autoclave is off by a little bit
- Set dial 2 notches below 134 °C.
6/29/2010
Started growing E. coli K12 cultures
- Poured 2 mL of LB broth into a Falcon tube
- Used strain of K12 from Dr. Lin's freezer
- Placed in incubator shaker for 24 hrs.
Added and inventoried supplies from Dr. Pinto's lab.
- Regular trash can be thrown out by going down one floor, then going outside to the trash bins.
- Chemical wastes must be cleaned by calling OSEH.
6/30/2010
Cryopreserved stock of K12
- 1 mL located in the iGEM box in the Lin lab.
- 1 mL located in the lab freezer.
7/1/2010
Cryopreserved DH5alpha according to protocol procedure on 6/30/2010
- Put 1 stock in -20C fridge in 1239
- Put the other stock in the -80C fridge in the Lin lab
