Team:UNIPV-Pavia/Calendar/June

From 2010.igem.org

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<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 24th|June, 24th]]</td><td align="left">14° Bio-Lab - Sara, Susanna Z., Alessandro.<br></td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 24th|June, 24th]]</td><td align="left">14° Bio-Lab - Sara, Susanna Z., Alessandro.<br></td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 25th|June, 25th]]</td><td align="left">15° Bio-Lab - Susanna Z., Chiara, Riccardo<br></td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 25th|June, 25th]]</td><td align="left">15° Bio-Lab - Susanna Z., Chiara, Riccardo<br></td></tr>
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<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 28th|June, 28th]]</td><td align="left">16° Bio-Lab - Susanna Z., Nicolò, Federica, Giacomo<br></td></tr>
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<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 29th|June, 29th]]</td><td align="left">17° Bio-Lab - Susanna Z., Nicolò, Riccardo,Giacomo, Lorenzo<br></td></tr>
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<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 30th|June, 30th]]</td><td align="left">18° Bio-Lab - Susanna Z.<br></td></tr>
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Revision as of 12:59, 2 July 2010
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JUNE


DATEACTIVITY
June, 3rd9° Meeting - Discussion of final and technical details about The Project. Planning of Bio-Lab activity.
June, 7th<partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in E. coli DH5alpha.
Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".
June, 8thInoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep
June, 9thMiniPrep of <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo>. Digestion, gel run/cut and ligation of:
  • I0: <partinfo>BBa_K125500</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
  • I1: <partinfo>BBa_E2050</partinfo> (E-S)+<partinfo>BBa_K165018</partinfo> (E-X)
  • I2: <partinfo>BBa_P1004</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
  • I3: <partinfo>BBa_K081008</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
June, 10thTransformation of ligations I0, I1, I2, I3 in home made E. coli DH5-alpha competent cells. Transformants were plated on LB+Amp agar plates
June, 11thInoculum from plates in liquid LB and glycerol stocks preparation for I0, I1, I2 and I3 in duplicate (2 colonies peaked from each plate).
June, 12thiGEM EU workshop
June, 13thiGEM EU workshop
June, 14th6° Bio-Lab - Lorenzo and Nicolò.
June, 15th7° Bio-Lab - Lorenzo and Manuel L., Sara and Alessandro.
June, 16th8° Bio-Lab - Susanna Z., Sara and Nicolò.
June, 17th9° Bio-Lab - Susanna Z., Nicolò and Riccardo.
June, 18th10° Bio-Lab - Susanna Z., Susanna S. and Federica.
June, 21st11° Bio-Lab - Nicolò, Manuel Z., Susanna Z.
June, 22nd12° Bio-Lab - Susanna Z., Lorenzo, Sara, Nicolò
June, 23rd13° Bio-Lab - Sara, Susanna Z., Nicolò.
June, 24th14° Bio-Lab - Sara, Susanna Z., Alessandro.
June, 25th15° Bio-Lab - Susanna Z., Chiara, Riccardo
June, 28th16° Bio-Lab - Susanna Z., Nicolò, Federica, Giacomo
June, 29th17° Bio-Lab - Susanna Z., Nicolò, Riccardo,Giacomo, Lorenzo
June, 30th18° Bio-Lab - Susanna Z.


CALENDAR
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April
May
June
July
August
September
October
November

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