Team:UNIPV-Pavia/Calendar/June

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Revision as of 09:28, 2 July 2010

JUNE

DATE	ACTIVITY
June, 3rd	9° Meeting - Discussion of final and technical details about The Project. Planning of Bio-Lab activity.
June, 7th	<partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in E. coli DH5alpha. Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".
June, 8th	Inoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep
June, 9th	MiniPrep of <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo>. Digestion, gel run/cut and ligation of: I0: <partinfo>BBa_K125500</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X) I1: <partinfo>BBa_E2050</partinfo> (E-S)+<partinfo>BBa_K165018</partinfo> (E-X) I2: <partinfo>BBa_P1004</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X) I3: <partinfo>BBa_K081008</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
June, 10th	Transformation of ligations I0, I1, I2, I3 in home made E. coli DH5-alpha competent cells. Transformants were plated on LB+Amp agar plates
June, 11th	Inoculum from plates in liquid LB and glycerol stocks preparation for I0, I1, I2 and I3 in duplicate (2 colonies peaked from each plate).
June, 12th	iGEM EU workshop
June, 13th	iGEM EU workshop
June, 14th	6° Bio-Lab - Lorenzo and Nicolò.
June, 15th	7° Bio-Lab - Lorenzo and Manuel L., Sara and Alessandro.
June, 16th	8° Bio-Lab - Susanna Z., Sara and Nicolò.
June, 17th	9° Bio-Lab - Susanna Z., Nicolò and Riccardo.
June, 18th	10° Bio-Lab - Susanna Z., Susanna S. and Federica.
June, 21st	11° Bio-Lab - Nicolò, Manuel Z., Susanna Z.
June, 22nd	12° Bio-Lab - Susanna Z., Lorenzo, Sara, Nicolò
June, 23rd	13° Bio-Lab - Sara, Susanna Z., Nicolò.
June, 24th	14° Bio-Lab - Sara, Susanna Z., Alessandro.
June, 25th	15° Bio-Lab - Susanna Z., Chiara, Riccardo

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 <table class=cont border="0" width="30%" align="center" valign="top">
 <tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr>
-<tr><td align="center" valign="top"><html><a name="June_3rd"></a></html>June, 3th</td><td align="left">9° Meeting - Discussion of final and technical details about [[Team:UNIPV-Pavia/Project|The Project]]. Planning of Bio-Lab activity.</td></tr>
+<tr><td align="center" valign="top"><html><a name="June_3rd"></a></html>June, 3rd</td><td align="left">9° Meeting - Discussion of final and technical details about [[Team:UNIPV-Pavia/Project|The Project]]. Planning of Bio-Lab activity.</td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 7th|June, 7th]]</td><td align="left"><partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in ''E. coli'' DH5alpha.<br>Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".</td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 8th|June, 8th]]</td><td align="left">Inoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep<br></td></tr>