Team:Alberta/Notebook

From 2010.igem.org

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<div id="Content">
<div id="Content">
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<h2>The Lab Notebook</h2>
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<div id="vtab0">
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  <li onclick="showTab('vtab1')">iGEM 2010 Notebook</li>
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  <li onclick="showTab('vtab2')">Building Parts</li>
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  <li onclick="showTab('vtab3')">Testing Parts</li>
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  <li onclick="showTab('vtab4')">Assembly Method</li>
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  <li onclick="showTab('vtab5')">Plates</li>
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  <li onclick="showTab('vtab6')">Competent Cells</li>
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  <li onclick="showTab('vtab7')">Software</li>
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<div id="vtab1" class="vtInfo"><span class="h2">iGEM 2010 Notebook</span>
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<br>
<p>The lab notebook chronicles our journey in the creation of the Genomikon kit.  Many paths were woven together in space and time to reach this finished masterpiece.  To help you navigate through these trials with us we have laid out our notebook in a layered fashion.  This page gives a sketch of each project and how it interacts with each other.  Then follow the links to a projects page for time line of the major landmarks and accomplishments.  If you require more details on the project the links within that page will take you to our day-by-day work log.</p>
<p>The lab notebook chronicles our journey in the creation of the Genomikon kit.  Many paths were woven together in space and time to reach this finished masterpiece.  To help you navigate through these trials with us we have laid out our notebook in a layered fashion.  This page gives a sketch of each project and how it interacts with each other.  Then follow the links to a projects page for time line of the major landmarks and accomplishments.  If you require more details on the project the links within that page will take you to our day-by-day work log.</p>
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<h3>Building Parts</h3>
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<div id="vtab2" class="vtInfo"><span class="h2">Building Parts</span>  
<p>The Building Parts project was responsible to first build a plasmid (plasmid 01)that contained our own specialized prefix and suffix nested inside of the standard BioBrick prefix and suffix.  After plasmid 01 existed we inserted the CcdB gene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02).  Plasmid 02 is fantastic base plasmid from which we are able to amplify any part at all because it provides a positive selection marker when transformed into DH5&alpha;. At this point we were able to make parts en masse to put in our kit. After obtaining a particular part in a plasmid we PCRed the part and digested it ready to use in Assembly or to Test the plasmid.</p>
<p>The Building Parts project was responsible to first build a plasmid (plasmid 01)that contained our own specialized prefix and suffix nested inside of the standard BioBrick prefix and suffix.  After plasmid 01 existed we inserted the CcdB gene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02).  Plasmid 02 is fantastic base plasmid from which we are able to amplify any part at all because it provides a positive selection marker when transformed into DH5&alpha;. At this point we were able to make parts en masse to put in our kit. After obtaining a particular part in a plasmid we PCRed the part and digested it ready to use in Assembly or to Test the plasmid.</p>
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<h3>Testing Parts</h3>
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<div id="vtab3" class="vtInfo"><span calss="h2">Testing Parts</span>
<p>Before we were able to test parts we created 2 base testing plasmids (vector 01 and vector 02).  Vector 01 is designed to test Open Reading Frame parts, or parts that code for proteins.  The part is flanked by a promoter and the start codon on one side and a stop codon and terminator on the other.  Vector 02 is designed to test linker parts, or parts that control the expression of the Open Reading Frame parts they are next two.  In Vector 02 the part is flanked by two distinct reporter genes, that by comparing the relative expression of the 2 reporter genes we can determine the behavior of the linking part.</p>
<p>Before we were able to test parts we created 2 base testing plasmids (vector 01 and vector 02).  Vector 01 is designed to test Open Reading Frame parts, or parts that code for proteins.  The part is flanked by a promoter and the start codon on one side and a stop codon and terminator on the other.  Vector 02 is designed to test linker parts, or parts that control the expression of the Open Reading Frame parts they are next two.  In Vector 02 the part is flanked by two distinct reporter genes, that by comparing the relative expression of the 2 reporter genes we can determine the behavior of the linking part.</p>
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<h3>Assemply Method</h3>
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<img src="https://static.igem.org/mediawiki/2010/b/b1/Alberta_Oscar.png" width="400px" height="533px" padding="0px" align="left"></img>
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<span class="h2">Oscar Cortes</span><br>
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<span class="h3">Specialized in Molecular Genetics (Graduate)</span>
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<div id="highlightb"></div>

Revision as of 04:00, 2 July 2010

genomikon

  • iGEM 2010 Notebook
  • Building Parts
  • Testing Parts
  • Assembly Method
  • Plates
  • Competent Cells
  • Software
    • iGEM 2010 Notebook

    The lab notebook chronicles our journey in the creation of the Genomikon kit. Many paths were woven together in space and time to reach this finished masterpiece. To help you navigate through these trials with us we have laid out our notebook in a layered fashion. This page gives a sketch of each project and how it interacts with each other. Then follow the links to a projects page for time line of the major landmarks and accomplishments. If you require more details on the project the links within that page will take you to our day-by-day work log.

    Building Parts

    The Building Parts project was responsible to first build a plasmid (plasmid 01)that contained our own specialized prefix and suffix nested inside of the standard BioBrick prefix and suffix. After plasmid 01 existed we inserted the CcdB gene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which we are able to amplify any part at all because it provides a positive selection marker when transformed into DH5α. At this point we were able to make parts en masse to put in our kit. After obtaining a particular part in a plasmid we PCRed the part and digested it ready to use in Assembly or to Test the plasmid.

    Testing Parts

    Before we were able to test parts we created 2 base testing plasmids (vector 01 and vector 02). Vector 01 is designed to test Open Reading Frame parts, or parts that code for proteins. The part is flanked by a promoter and the start codon on one side and a stop codon and terminator on the other. Vector 02 is designed to test linker parts, or parts that control the expression of the Open Reading Frame parts they are next two. In Vector 02 the part is flanked by two distinct reporter genes, that by comparing the relative expression of the 2 reporter genes we can determine the behavior of the linking part.

    Oscar Cortes
    Specialized in Molecular Genetics (Graduate)


    page78


    Building Parts Testing Parts Assembly Method Competent Cells Plates Kit Manual Software
    Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety
    Retrieved from "http://2010.igem.org/Team:Alberta/Notebook"