"

From 2010.igem.org

Revision as of 17:33, 25 October 2010 (view source) Jesús (Talk | contribs) ← Older edit		Revision as of 17:38, 25 October 2010 (view source) Jesús (Talk | contribs) Newer edit →
Line 25:		Line 25:
	==''Tuesday''==		==''Tuesday''==
-	'''With a overnight inoculum  we going did a miniprep.'''	+	'''With a overnight inoculum  we did a miniprep.'''
	'''The miniprep was done with a Quiagen Kit.'''		'''The miniprep was done with a Quiagen Kit.'''
Line 67:		Line 67:
-	[[Image:Noncon.gif|500px|thumb|left]]	+	[[Image:Noncon.gif|300px|thumb|left]] [[Image:Con1.gif|300px|Right]]

---

Revision as of 17:38, 25 October 2010

Contents 1 Week #5 2 04th October - 08 October 2010 2.1 Monday 2.2 Tuesday 2.3 Wensday

Week #5

04th October - 08 October 2010

Monday

• Let's picked up colonys our transformant cells and prepare all to do miniprep.

For this we used 30ml de LB liquid agar medium per falcon tube at 80μ/ml

Cloramphenicol concentration we left overnight culture to 37º

Tuesday

With a overnight inoculum we did a miniprep.

The miniprep was done with a Quiagen Kit.

Then we going to do a duble digest with EcoRI and PstI to check out our ligations.

DNA	5μl
Buffer NB2	1μl
EcoRI	0.5μl
PstI	0.5μl
H2O	2.7μl
BSA	0.3μl
Total	10μl

Wensday

We run a gel to confirm our ligations as follow

• PCR 1 Lig S with Psb1C3 Confirmed
• PCR 2 Lig S with Psb1C3 Non confirmed
• PCR 3 Lig S with Psb1C3 Confirmed
• PCR 4 Lig C with Psb1C3 Non confirmed