Revision as of 16:24, 25 October 2010

Week #5

Let's picked up colonys our transformant cells and prepare all to do miniprep.

For this we used 30ml de LB liquid agar medium per falcon tube at 80μ/ml

Cloramphenicol concentration we left overnight culture to 37º

With a overnight inoculum we going to do a miniprep.

The miniprep was done with a Quiagen Kit.

Then we going to do a duble digest with EcoRI and PstI to check out our ligations.

Revision as of 16:23, 25 October 2010 (view source) Jesús (Talk \| contribs) ← Older edit		Revision as of 16:24, 25 October 2010 (view source) Jesús (Talk \| contribs) Newer edit →
Line 17:		Line 17:


-	[[Image:Labo_271.JPG\|500px]] [[Image:DSC02107.JPG\|500px]]	+	[[Image:Labo_271.JPG\|500px]]
		+
		+	[[Image:DSC02107.JPG\|500px]]