User:MayaBrandi/1 July 2010

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(Preparations for PPI study of the differentially expressed proteins in melanocytes)
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1. microarrays - reliability
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1 Microarrays - reliability
         - How reliable is the protein regulation profile in the article "Transcriptional profiling of melanocytes..."
         - How reliable is the protein regulation profile in the article "Transcriptional profiling of melanocytes..."

Revision as of 15:15, 1 July 2010

1 Microarrays - reliability 

       - How reliable is the protein regulation profile in the article "Transcriptional profiling of melanocytes..."

2 How should one priorate different types of protein-protein interactions? 

   Focus on experimental (not predictive ) data and focus first of all at physical PPI second at co expression and co localisation. Skip the rest.
   
   To improve PPI reliability
     * Find a good coverage from many primary databases. -How many and with?
     * What experiments were used? If the same result is dereved from many different experiments then the PPI is more reliable.
     * Look at 3D structures...?
   Compare with canonical pathways to get a better insight in the functional roles of the proteins in the network.
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