Revision as of 14:25, 25 October 2010

BioBrick Toolbox

BioBrick assembly A-parts are used for N-Terminal and C-parts for C-terminal fusion. B-part is the linker that connects the two entities.

To construct the aimed fusion proteins, we first blunt-end cloned our BioBricks into the storage vector pSEVA132 <partinfo>BBa_K422002</partinfo> (Victor de Lorenzo's lab, KanR, pBBR1 ori). All parts are sequence verified and compatible with BBF RFC28 (http://dspace.mit.edu/handle/1721.1/46721).

Check out our general scheme for the easy construction of Tom Knight's OAS and BBF RFC28 compatible working vectors [1]!

Chemotaxis BrickBox

Name	Insert	Orientation	Name	Insert	Orientation	Description
<partinfo>BBa_K422003</partinfo>	CheB	A-part	<partinfo>BBa_K422004</partinfo>	CheB	C-part	Demthylase: Demethylates methyl accepting chemotaxis proteins MCPs
<partinfo>BBa_K422005</partinfo>	CheR	A-part	<partinfo>BBa_K422006</partinfo>	CheR	C-part	Methyltransferase: Methylates methyl accepting chemotaxis proteins MCPs
<partinfo>BBa_K422007</partinfo>	CheY	A-part	<partinfo>BBa_K422008</partinfo>	CheY	C-part	Induces tumbling by interacting with the flagellar switch protein FliM

Anchor BrickBox

Name	Insert	Orientation	Description
<partinfo>BBa_K422009</partinfo>	TetR	A-part	Tetracyclin repressor: Binds to its operator tetO for spatial localization of a fusion protein to the DNA.
<partinfo>BBa_K422010</partinfo>	trigA	A-part	Ribosome binding domain of the trigger factor: Binds to the large subunit of the ribosome.
<partinfo>BBa_K422011</partinfo>	MreB	C-part	Prokaryotic actin homologue: Assembles into helical filaments underneath the cytoplasmic membrane.

Ligth sensor BrickBox

Name	Insert	Orientation	Description	Favourite part
<partinfo>BBa_K422012</partinfo>	Pif3	C-part	Phytochrome interacting factor: N-Terminus of Pif3 selectively binds the Prf form of the phytochrome and rapidly dissociates in response to reconversion to the Pr state.
<partinfo>BBa_K422013</partinfo>	PhyB	C-part	Phytochromes type II: Exists in an biologically inactive form Pr absorbing red light and an active configuration Pfr which absorbs far-red light.

Reporter BrickBox

Name	Insert	Orientation	Description	Favourite part
<partinfo>BBa_K422014</partinfo>	cyPet	A-part	CFP variant.

@@ Line 3: / Line 3: @@
 = BioBrick Toolbox =
-To construct the aimed fusion proteins, we first cloned our BioBricks into the storage vector pSEVA132 <partinfo>BBa_K422002</partinfo> (Victor de Lorenzo's lab, KanR, pBBR1 ori). All parts are sequence verified and compatible with BBF RFC28 (http://dspace.mit.edu/handle/1721.1/46721).
+[[Image:Apart_Cpart.png|thumb|500px|'''BioBrick assembly''' A-parts are used for N-Terminal and C-parts for C-terminal fusion. B-part is the linker that connects the two entities.]]
-A-parts N-Terminal fusion and C-parts C-terminal fusion. B-part is the linker that connects the two entities.
+To construct the aimed fusion proteins, we first blunt-end cloned our BioBricks into the storage vector pSEVA132 <partinfo>BBa_K422002</partinfo> (Victor de Lorenzo's lab, KanR, pBBR1 ori).
+All parts are sequence verified and compatible with BBF RFC28 (http://dspace.mit.edu/handle/1721.1/46721).
 Check out our general scheme for the easy construction of Tom Knight's OAS and BBF RFC28 compatible working vectors [https://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning]!
 == Chemotaxis BrickBox ==

Team:ETHZ Basel/Achievements/BioBrick Toolbox

From 2010.igem.org