Igem2010/Main/synthetic miR Kit/September

From 2010.igem.org

(Difference between revisions)
(New page: ===23/09/2010=== *dual luciferase assay ** all constructs are functional as firefly and renilla luciferase are expressed ** transfection of construct into HEK 293 T-REx cells reveals that ...)
(23/09/2010)
Line 3: Line 3:
** all constructs are functional as firefly and renilla luciferase are expressed
** all constructs are functional as firefly and renilla luciferase are expressed
** transfection of construct into HEK 293 T-REx cells reveals that the CMVTetO2 (construct K4) is also functional as the Firefly luciferase is not expressed
** transfection of construct into HEK 293 T-REx cells reveals that the CMVTetO2 (construct K4) is also functional as the Firefly luciferase is not expressed
 +
[[Image:22092010_DLA_promoters]]

Revision as of 14:19, 25 October 2010

23/09/2010

  • dual luciferase assay
    • all constructs are functional as firefly and renilla luciferase are expressed
    • transfection of construct into HEK 293 T-REx cells reveals that the CMVTetO2 (construct K4) is also functional as the Firefly luciferase is not expressed

File:22092010 DLA promoters