Team:UNIPV-Pavia/Calendar/October/settimana3

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Revision as of 09:53, 25 October 2010

OCTOBER: WEEK 3

October, 18th

We have finally completed the parts transfer to <partinfo>pSB1C3</partinfo> plasmid. All the parts that will be sent to the registry were previously screened by UNIPV-Pavia TEAM, as reported in this notebook and all of them were sequenced and sequencing results were correct.

After parts have been moved to the shipping plasmid and checked by agarose gel electrophoresis.

The parts we sent to MIT and submitted to the registry are:

Part name	Wiki name	Plasmid	Number
<partinfo>BBa_K300005</partinfo>	I0	pSB1C3	1
<partinfo>BBa_K300006</partinfo>	I1	pSB1C3	2
<partinfo>BBa_K300009</partinfo>	I3	pSB1C3	3
<partinfo>BBa_K300008</partinfo>	I5	pSB1C3	4
<partinfo>BBa_K300024</partinfo>	I7	pSB1C3	5
<partinfo>BBa_K300019</partinfo>	I12	pSB1C3	6
<partinfo>BBa_K300030</partinfo>	I14	pSB1C3	7
<partinfo>BBa_K300028</partinfo>	I15	pSB1C3	8
<partinfo>BBa_K300029</partinfo>	I16	pSB1C3	9
<partinfo>BBa_K300025</partinfo>	I17	pSB1C3	10
<partinfo>BBa_K300026</partinfo>	I18	pSB1C3	11
<partinfo>BBa_K300027</partinfo>	I19	pSB1C3	12
<partinfo>BBa_K300002</partinfo>	I20	pSB1C3	13
<partinfo>BBa_K300003</partinfo>	I21	pSB1C3	14
<partinfo>BBa_K300031</partinfo>	I22	pSB1C3	15
<partinfo>BBa_K300081</partinfo>	I26	pSB1C3	16
<partinfo>BBa_K300079</partinfo>	I31	pSB1C3	17
<partinfo>BBa_K300080</partinfo>	I35	pSB1C3	18
<partinfo>BBa_K300086</partinfo>	I47	pSB1C3	19
<partinfo>BBa_K300088</partinfo>	I48	pSB1C3	20
<partinfo>BBa_K300090</partinfo>	I49	pSB1C3	21
<partinfo>BBa_K300082</partinfo>	I55	pSB1C3	22
<partinfo>BBa_K300084</partinfo>	I78	pSB1C3	23
<partinfo>BBa_K300083</partinfo>	I79	pSB1C3	24
<partinfo>BBa_K300010</partinfo>	I80	pSB1C3	25
<partinfo>BBa_K300007</partinfo>	I70 - BioBrick	<partinfo>BBa_K300001</partinfo>	26
<partinfo>BBa_K300007</partinfo>	I70 - vector	<partinfo>BBa_K300001</partinfo>	27
<partinfo>BBa_K300004</partinfo>	INTEIN	pSB1C3	28
<partinfo>BBa_I52002</partinfo>	I51	<partinfo>BBa_K300000</partinfo>	29

The parts we have in freezer and we only submitted to the Registry are:

Part name	Wiki name	Plasmid
<partinfo>BBa_K300021</partinfo>	I8	<partinfo>BBa_J61002</partinfo>
<partinfo>BBa_K300022</partinfo>	I9	<partinfo>BBa_J61002</partinfo>
<partinfo>BBa_K300023</partinfo>	I10	<partinfo>BBa_J61002</partinfo>
<partinfo>BBa_K300091</partinfo>	I63	<partinfo>pSB1AK3</partinfo>
<partinfo>BBa_K300092</partinfo>	I64	<partinfo>pSB1A2</partinfo>
<partinfo>BBa_K300099</partinfo>	I65	<partinfo>pSB1A2</partinfo>
<partinfo>BBa_K300980</partinfo>	YEAST MR.G	pMA commercial vector
<partinfo>BBa_K300982</partinfo>	CREAM	-
<partinfo>BBa_K300983</partinfo>	CRIM MR.G	pMK-RQ commercial vector

In order to obtain better results with screening PCR for the E. coli genome integration project, we perform amplifications using an annealing temperature of 63°C. Screening PCR was performed on MC42(A-B-C), MC43(A-B-C), MG42(B-C), MG43(A-B-C), MC1061 and MG1655. Primers used were BBa_K300975-BBa_K300976, BBa_K300976-BBa_K300977, BBa_K300977-BBa_K300978.

The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300975-BBa_K300976.