"
Team:Tsinghua/Notebook/26 July 2010
From 2010.igem.org
(Difference between revisions)
(→Module II, Danyang's part:) |
(→2c) |
||
| Line 1: | Line 1: | ||
| - | == 2c == | + | == Module I, Group 2c == |
| - | To test the effectivity of newly-synthetized, PCR assay to amplify mCherry, Kan and eGFP, Chlr is conducted. | + | To test the effectivity of newly-synthetized primer, PCR assay to amplify mCherry, Kan and eGFP, Chlr is conducted. |
PCR system (FastPFU): | PCR system (FastPFU): | ||
| Line 16: | Line 16: | ||
| - | PCR program | + | PCR program---- |
| + | |||
1.95℃ 1min | 1.95℃ 1min | ||
| Line 27: | Line 28: | ||
5.72℃ 5min | 5.72℃ 5min | ||
| - | |||
Latest revision as of 07:27, 25 October 2010
Module I, Group 2c
To test the effectivity of newly-synthetized primer, PCR assay to amplify mCherry, Kan and eGFP, Chlr is conducted.
PCR system (FastPFU):
H2O 31.5μl 5×buffer 10μl dNTP 5μl primer1 1μl primer2 1μl template 1μl FastPFU 0.5μl Total 50μl
PCR program----
1.95℃ 1min 2.95℃ 20s 3.53℃for eGFP 51℃for mCherry 20s 51℃for Kan&Chlr 4.72℃ 30s 30 cycles from 2 to 4 5.72℃ 5min
Result for PCR: After agarose gel is run, no specific bands are observed.
Conclusion: reaction condition for PCR is not ideal enough, thus we shall change the condition and try this again.
