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Team:Tsinghua/Notebook/27 July 2010
From 2010.igem.org
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Conduct PCR to amplify eGFP, Kan and Chlr with DNA polymerase pyrobest. | Conduct PCR to amplify eGFP, Kan and Chlr with DNA polymerase pyrobest. | ||
Revision as of 07:17, 25 October 2010
Module I, group 2(b)
Using the newly synthesized primers go through a pre-test experiment.
PCR system (FastPFU):
H2O 31.4μl 5×buffer 10μl dNTP 5μl primer1 1μl primer2 1μl template 1μl FastPFU 0.6μl Total 50μl
Group 2c
Conduct PCR to amplify eGFP, Kan and Chlr with DNA polymerase pyrobest.
PCR system (FastPFU):
H2O 36.7μl 10×buffer 5μl dNTP 5μl primer1 1μl primer2 1μl template 1μl FastPFU 0.3μl Total 50μl
result
The concentration of the M, K and C are a little lower. We’ll amplify them once again with two degrees lower of the annealing temperature.
