Team:Tsinghua/Notebook/16 August 2010

From 2010.igem.org

(Difference between revisions)
(New page: == Module I, DT and Fan's part: == Universal primers of pUC19 are available now (marked p-up and p-do)! Use the universal primers to detect PEM, PKC and PEMKC with SuperMix system shown b...)
(Module I, DT and Fan's part:)
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== Module I, DT and Fan's part: ==
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== Module I, group 2(b) ==
Universal primers of pUC19 are available now (marked p-up and p-do)! Use the universal primers to detect PEM, PKC and PEMKC with SuperMix system shown before.
Universal primers of pUC19 are available now (marked p-up and p-do)! Use the universal primers to detect PEM, PKC and PEMKC with SuperMix system shown before.
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== result ==
== result ==
The result shows that PEM plasmids are truly positive but kan gene were not ligated successfully. So that the EMKC are not positive with K missed.
The result shows that PEM plasmids are truly positive but kan gene were not ligated successfully. So that the EMKC are not positive with K missed.

Revision as of 06:55, 25 October 2010

Module I, group 2(b)

Universal primers of pUC19 are available now (marked p-up and p-do)! Use the universal primers to detect PEM, PKC and PEMKC with SuperMix system shown before.

result

The result shows that PEM plasmids are truly positive but kan gene were not ligated successfully. So that the EMKC are not positive with K missed.