Team:Tsinghua/Notebook/16 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: == Module I, DT and Fan's part: == Universal primers of pUC19 are available now (marked p-up and p-do)! Use the universal primers to detect PEM, PKC and PEMKC with SuperMix system shown b...) |
(→Module I, DT and Fan's part:) |
||
Line 1: | Line 1: | ||
- | == Module I, | + | == Module I, group 2(b) == |
Universal primers of pUC19 are available now (marked p-up and p-do)! Use the universal primers to detect PEM, PKC and PEMKC with SuperMix system shown before. | Universal primers of pUC19 are available now (marked p-up and p-do)! Use the universal primers to detect PEM, PKC and PEMKC with SuperMix system shown before. | ||
- | |||
== result == | == result == | ||
The result shows that PEM plasmids are truly positive but kan gene were not ligated successfully. So that the EMKC are not positive with K missed. | The result shows that PEM plasmids are truly positive but kan gene were not ligated successfully. So that the EMKC are not positive with K missed. |
Revision as of 06:55, 25 October 2010
Module I, group 2(b)
Universal primers of pUC19 are available now (marked p-up and p-do)! Use the universal primers to detect PEM, PKC and PEMKC with SuperMix system shown before.
result
The result shows that PEM plasmids are truly positive but kan gene were not ligated successfully. So that the EMKC are not positive with K missed.