Team:Mexico-UNAM-CINVESTAV/Notebook/Week Five

From 2010.igem.org

(Difference between revisions)
Line 22: Line 22:
==''Tuesday''==
==''Tuesday''==
 +
 +
'''With a inoculum overnight culture we going to do a miniprep'''
 +
 +
'''The miniprep was done with a Quiagen Kit'''
 +
 +
'''Then we going to do a duble digestion with EcoRI and PstI to check out our ligations'''
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 +
 +
{| border="1"
 +
|-
 +
|DNA
 +
|5μl
 +
|-
 +
|Buffer NB2
 +
|1μl
 +
|-
 +
|EcoRI
 +
|0.5μl
 +
|-
 +
|PstI
 +
|0.5μl
 +
|-
 +
|H2O
 +
|2.7μl
 +
|-
 +
|BSA
 +
|0.3μl
 +
|-
 +
|Total
 +
|10μl
 +
|}

Revision as of 06:28, 25 October 2010


Contents

Week #5

04th October - 08 October 2010

Monday

  • Let's picked up colonys our transformant cells and prepare all to do miniprep.

For this we used 30ml de LB liquid agar medium per falcon tube at 80μ/ml

Cloramphenicol concentration we left overnight culture to 37º


Labo 271.JPG


Tuesday

With a inoculum overnight culture we going to do a miniprep

The miniprep was done with a Quiagen Kit

Then we going to do a duble digestion with EcoRI and PstI to check out our ligations


DNA 5μl
Buffer NB2 1μl
EcoRI 0.5μl
PstI 0.5μl
H2O 2.7μl
BSA 0.3μl
Total 10μl
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