"
User:AndreasConstantinou/29 June 2010
From 2010.igem.org
(Difference between revisions)
(New page: == Expression of SOD and yCCS from [http://partsregistry.org/Part:BBa_K243033 pEX expression vector] == * Set a starter culture of DH5alpha cells transformed with pEX vectors containing S...) |
(→Expression of SOD and yCCS from pEX expression vector) |
||
| Line 1: | Line 1: | ||
| - | == Expression of SOD and yCCS from | + | == Expression of SOD and yCCS from pEX expression vector == |
| + | Two cell cultures of DH5alpha cells respectively transformed with pEX vectors carrying superoxidase dismutase (SOD) and yeast copper chaperone (yCCS) were previously prepared and received from Nina and Johan. | ||
| - | * | + | =====Procedures===== |
| - | ** 37°C, 250 rpm | + | * An ON starter culture was set by inoculating 5 ml LB + 100 ug/ml Amp with 5 ul of each culture. |
| + | ** 37°C, 250 rpm. | ||
| + | |||
| + | ''Continued 30/6'' | ||
Latest revision as of 16:00, 29 June 2010
Expression of SOD and yCCS from pEX expression vector
Two cell cultures of DH5alpha cells respectively transformed with pEX vectors carrying superoxidase dismutase (SOD) and yeast copper chaperone (yCCS) were previously prepared and received from Nina and Johan.
Procedures
- An ON starter culture was set by inoculating 5 ml LB + 100 ug/ml Amp with 5 ul of each culture.
- 37°C, 250 rpm.
Continued 30/6
