Team:MIT phage background
From 2010.igem.org
(Difference between revisions)
Line 62: | Line 62: | ||
<img style="float: left" src="https://static.igem.org/mediawiki/2010/e/ed/P3_image.jpg" height=400px title="Image from: Arap, Phage Display Technology – Applications and Innovations, 2005"> | <img style="float: left" src="https://static.igem.org/mediawiki/2010/e/ed/P3_image.jpg" height=400px title="Image from: Arap, Phage Display Technology – Applications and Innovations, 2005"> | ||
<br> | <br> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
<b>M13 ANATOMY</b> | <b>M13 ANATOMY</b> | ||
+ | <br> | ||
+ | M13 bacteriophage is a long, filamentous virus that infects bacteria via interactions with the F-pilus. Below is a list of genes: | ||
<br> | <br> | ||
<ul><li> | <ul><li> | ||
Line 91: | Line 85: | ||
PV – dimerizes and binds ssDNA, packaging it into a rod for assembly</li></ul> | PV – dimerizes and binds ssDNA, packaging it into a rod for assembly</li></ul> | ||
<br> | <br> | ||
- | |||
<br> | <br> | ||
<img src="https://static.igem.org/mediawiki/2010/b/be/M13_parts.png" title="Image from: Barbas III et al. Phage Display: a Laboratory Manual. 2004." height=200px> | <img src="https://static.igem.org/mediawiki/2010/b/be/M13_parts.png" title="Image from: Barbas III et al. Phage Display: a Laboratory Manual. 2004." height=200px> | ||
Line 107: | Line 100: | ||
Termination: PIII/PXI are added to the end of the particle | Termination: PIII/PXI are added to the end of the particle | ||
</li></ul> | </li></ul> | ||
+ | <br> | ||
+ | <b>PHAGE DISPLAY</b> | ||
+ | <br> | ||
+ | By genetically modifying the phage, fusion proteins can be created that become integrated into the phage coat in a defined manner. Usually these new proteins are linked to the pIII or pVIII coat proteins (though PVII or PIX can be used). Thus, novel proteins can be displayed on the phage coat. This has historically been used as a mechanism for screening libraries of proteins or peptides (e.g., antibodies) for binding to a specific substrate or ligand of interest. In a process called panning, a population of phage is iteratively enriched for those that bind a substrate. Phage with proteins that do not bind are washed away and the remaining phage can then be amplified through infection. | ||
+ | <br><br> | ||
+ | <b>HYPERPHAGE</b> | ||
+ | <br> | ||
+ | Hyperphage is a plasmid with the gene for pIII truncated. The pIII protein is required for phage exit from the host cell membrane; phage without a proper pIII grow into long fibril-like structures called polyphage. The goal is to crosslink these polyphage. | ||
+ | <br><br> | ||
<div style="text-align:center"> | <div style="text-align:center"> | ||
← <a href="https://2010.igem.org/Team:MIT_phage">Introduction</a> | ← <a href="https://2010.igem.org/Team:MIT_phage">Introduction</a> |
Revision as of 00:49, 25 October 2010
hairy cells and polymerizing phage - background |
M13 ANATOMY M13 bacteriophage is a long, filamentous virus that infects bacteria via interactions with the F-pilus. Below is a list of genes:
M13 ASSEMBLY
PHAGE DISPLAY By genetically modifying the phage, fusion proteins can be created that become integrated into the phage coat in a defined manner. Usually these new proteins are linked to the pIII or pVIII coat proteins (though PVII or PIX can be used). Thus, novel proteins can be displayed on the phage coat. This has historically been used as a mechanism for screening libraries of proteins or peptides (e.g., antibodies) for binding to a specific substrate or ligand of interest. In a process called panning, a population of phage is iteratively enriched for those that bind a substrate. Phage with proteins that do not bind are washed away and the remaining phage can then be amplified through infection. HYPERPHAGE Hyperphage is a plasmid with the gene for pIII truncated. The pIII protein is required for phage exit from the host cell membrane; phage without a proper pIII grow into long fibril-like structures called polyphage. The goal is to crosslink these polyphage.
← Introduction
Design →
|